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Series GSE60270 Query DataSets for GSE60270
Status Public on Oct 09, 2014
Title Enhancer Activation Requires Trans-Recruitment of a Mega Transcription Factor Complex (ChIP-seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Enhancers provide critical information directing cell-type specific transcriptional programs, regulated by binding of signal-dependent transcription factors and their associated cofactors. Here we report that the most strongly activated estrogen (E2)-responsive enhancers are characterized by trans-recruitment and in situ assembly of a large 1-2MDa complex of diverse DNA-binding transcription factors by ERĪ± at ERE-containing enhancers. We refer to enhancers recruiting these factors as mega transcription factor-bound in trans (MegaTrans) enhancers. The MegaTrans complex is a signature of the most potent functional enhancers and is required for activation of enhancer RNA transcription and recruitment of coactivators, including p300 and Med1. The MegaTrans complex functions, in part, by recruiting specific enzymatic machinery, exemplified by DNA-dependent protein kinase. Thus, MegaTrans-containing enhancers represent a cohort of functional enhancers that mediate a broad and important transcriptional program and provide a molecular explanation for transcription factor clustering and hotspots noted in the genome.
Overall design Chromatin Immunoprecipitation (ChIP) assay followed by high throughput sequencing (ChIP-seq)
Contributor(s) Liu Z, Merkurjev D, Rosenfeld MG
Citation(s) 25303530
Submission date Aug 08, 2014
Last update date May 15, 2019
Contact name Daria Merkurjev
Phone 858-534-5858
Organization name UCSD
Department Medicine
Lab Michael G. Rosenfeld Laboratory
Street address 9500 Gilman Drive, Mail Code 0648
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
Platforms (2)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (48)
GSM1469979 ERa_ChIPseq_Veh_Exp1
GSM1469980 ERa_ChIPseq_E2_Exp1
GSM1469981 RARa_WT_BiotinChIPseq_noDox_Exp2
This SubSeries is part of SuperSeries:
GSE60272 Enhancer Activation Requires Trans-Recruitment of a Mega Transcription Factor Complex
BioProject PRJNA257921
SRA SRP045390

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Supplementary file Size Download File type/resource
GSE60270_RAW.tar 11.8 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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