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Series GSE60221 Query DataSets for GSE60221
Status Public on Aug 09, 2014
Title The Nuclear Exosome is Active and Important during Budding Yeast Meiosis
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Summary We have analysed the activity of the nuclear exosome during meiosis by deletion of TRF4, which encodes a key component of the exosome targeting complex TRAMP. We find that TRAMP mutants produce high levels of CUTs during meiosis that are undetectable in wild-type cells, showing that the nuclear exosome remains functional for CUT degradation. Lack of TRAMP activity stabilises ~1600 CUTs in meiotic cells, which occupy 40% of the binding capacity of the nuclear cap binding complex (CBC).
Overall design One sample each of Cbc2-associated RNA from wild-type and trf4-deleted cells at 6 hours of meiosis
Contributor(s) Frenk S, Oxley D, Houseley J
Citation(s) 25210768
Submission date Aug 08, 2014
Last update date May 15, 2019
Contact name Jonathan Houseley
Organization name Babraham Institute
Department Epigenetics Programme
Street address Babraham Institute
City Babraham
State/province Cambridgeshire
ZIP/Postal code CB22 3AT
Country United Kingdom
Platforms (1)
GPL13272 Illumina Genome Analyzer IIx (Saccharomyces cerevisiae)
Samples (2)
GSM1467865 Wild type
GSM1467866 trf4 deletion
BioProject PRJNA257783
SRA SRP045356

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Supplementary file Size Download File type/resource
GSE60221_100bp_quant_for_GEO.txt.gz 1.3 Mb (ftp)(http) TXT
GSE60221_ORF_analysis_log_quant_for_GEO.txt.gz 308.8 Kb (ftp)(http) TXT
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