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Series GSE60138 Query DataSets for GSE60138
Status Public on Dec 26, 2014
Title SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Specification of primordial germ cells (PGCs) marks the beginning of the totipotent state. However, without a tractable experimental model, the mechanism of human PGC (hPGC) specification remains unclear. Here, we demonstrate specification of hPGC-like cells (hPGCLCs) from germline competent pluripotent stem cells. The characteristics of hPGCLCs are consistent with the embryonic hPGCs and a germline seminoma that share a CD38 cell-surface marker, which collectively defines likely progression of the early human germline. Remarkably, SOX17 is the key regulator of hPGC-like fate, whereas BLIMP1 represses endodermal and other somatic genes during specification of hPGCLCs. Notable mechanistic differences between mouse and human PGC specification could be attributed to their divergent embryonic development and pluripotent states, which might affect other early cell-fate decisions. We have established a foundation for future studies on resetting of the epigenome in hPGCLCs and hPGCs for totipotency and the transmission of genetic and epigenetic information.
 
Overall design RNA-Seq analysis to investigate transcriptomes of hPGC-like cells (hPGCLCs), fetal hPGCs, TCam-2 and hESCs
 
Contributor(s) Irie N, Weinberger L, Tang WW, Kobayashi T, Viukov S, Manor Y, Dietmann S, Hanna JH, Surani MA
Citation(s) 25543152
Submission date Aug 06, 2014
Last update date May 15, 2019
Contact name Sabine Dietmann
Organization name Wellcome Trust/Medical Research Council Stem Cell Institute
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QR
Country United Kingdom
 
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (16)
GSM1466229 TCam-2 rep1
GSM1466230 TCam-2 rep2
GSM1466233 hPGC male week 7 rep1
Relations
BioProject PRJNA257553
SRA SRP045294

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60138_RAW.tar 3.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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