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| Status |
Public on Dec 12, 2014 |
| Title |
Browning of human adipocytes requires KLF11 and reprogramming of PPARγ super-enhancers |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Here we have characterized the transcriptional processes underlying the formation of human brown in white (i.e. brite) adipocytes using a genome-wide approach. We show that the browning process is associated with reprogramming of peroxisome proliferator-activated receptor γ (PPARγ) binding to form brite adipocyte-selective PPARγ super-enhancers that appear to play a key role in activation of brite adipocyte-selective genes. We identify the KLF11 gene based on its association with a PPARγ super-enhancer and show that KLF11 is a novel browning factor directly induced by rosiglitazone and required for the activation of brite adipocyte-selective gene program by rosiglitazone.
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| Overall design |
Genome-wide profiling of Dnase I hypersenstive (DHS) sites, epigenomic marks, transcription factor and co-factor binding, and gene expression in hMADS white and brite adipocytes
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| Contributor(s) |
Loft A, Mandrup S |
| Citation(s) |
25504365 |
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| Submission date |
Jul 23, 2014 |
| Last update date |
Feb 21, 2024 |
| Contact name |
Anne Loft |
| E-mail(s) |
anlo@bmb.sdu.dk
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| Organization name |
University of Southern Denmark
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| Department |
Biochemistry & Molecular Biology
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| Street address |
Campusvej 55
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| City |
Odense M |
| ZIP/Postal code |
5230 |
| Country |
Denmark |
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| Platforms (2) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (36)
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| GSM1443804 |
CBP hMADS brite, day 19 |
| GSM1443805 |
H3K27ac hMADS white rep1, day 19 |
| GSM1443806 |
H3K27ac hMADS brite rep1, day 19 |
| GSM1443807 |
H3K27ac hMADS white rep2, day 19 |
| GSM1443808 |
H3K27ac hMADS brite rep2, day 19 |
| GSM1443809 |
PPARgamma hMADS white rep1, day 19 |
| GSM1443810 |
PPARgamma hMADS brite rep1, day 19 |
| GSM1443811 |
PPARgamma hMADS white rep2, day 19 |
| GSM1443812 |
PPARgamma hMADS brite rep2, day 19 |
| GSM1443813 |
KLF11 hMADS white rep1, day 19 |
| GSM1443814 |
KLF11 hMADS brite rep1, day 19 |
| GSM1443815 |
KLF11 hMADS white rep2, day 19 |
| GSM1443816 |
KLF11 hMADS brite rep2, day 19 |
| GSM1443817 |
Input hMADS white rep1, day 19 |
| GSM1443818 |
Input hMADS brite rep1, day 19 |
| GSM1443819 |
RNA hMADS rep1, day 0 |
| GSM1443820 |
RNA hMADS rep2, day 0 |
| GSM1443821 |
RNA hMADS white rep 1, day 19 |
| GSM1443822 |
RNA hMADS white rep 2, day 19 |
| GSM1443823 |
RNA hMADS white rep 3, day 19 |
| GSM1443824 |
RNA hMADS brite rep 1, day 19 |
| GSM1443825 |
RNA hMADS brite rep 2, day 19 |
| GSM1443826 |
RNA hMADS brite rep 3, day 19 |
| GSM1443827 |
RNA hMADS, Scramble rep1, Day 16 |
| GSM1443828 |
RNA hMADS, KLF11 KD rep 1, Day 16 |
| GSM1443829 |
RNA hMADS, Scramble rep2, Day 16 |
| GSM1443830 |
RNA hMADS, KLF11 KD rep 2, Day 16 |
| GSM1443831 |
H3K27ac hMADS Scramble, Day 16 |
| GSM1443832 |
H3K27ac hMADS, KLF11 KD, Day 16 |
| GSM1537677 |
MED1 hMADS white rep1, day 19 |
| GSM1537678 |
MED1 hMADS brite rep1, day 19 |
| GSM1537679 |
MED1 hMADS white rep2, day 19 |
| GSM1537680 |
MED1 hMADS brite rep2, day 19 |
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| Relations |
| BioProject |
PRJNA255992 |
| SRA |
SRP044756 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE59703_AllPPARgSuperenhancers.bed.gz |
16.2 Kb |
(ftp)(http) |
BED |
| GSE59703_BriteselectivePPARgPeaks.bed.gz |
31.8 Kb |
(ftp)(http) |
BED |
| GSE59703_BriteselectivePPARgSuperenhancers.bed.gz |
4.2 Kb |
(ftp)(http) |
BED |
| GSE59703_Briteselectivegenes.txt.gz |
148.0 Kb |
(ftp)(http) |
TXT |
| GSE59703_ConstantPPARgPeaks.bed.gz |
34.4 Kb |
(ftp)(http) |
BED |
| GSE59703_ConstantPPARgSuperenhancers.bed.gz |
2.0 Kb |
(ftp)(http) |
BED |
| GSE59703_Constitutivegenes.txt.gz |
82.8 Kb |
(ftp)(http) |
TXT |
| GSE59703_KLF11KDnormalizedlog2readsperkilobase.txt.gz |
341.6 Kb |
(ftp)(http) |
TXT |
| GSE59703_KLF11regulatedgenes.txt.gz |
126.6 Kb |
(ftp)(http) |
TXT |
| GSE59703_Normalizedlog2readsperkilobase.txt.gz |
1.8 Mb |
(ftp)(http) |
TXT |
| GSE59703_RAW.tar |
823.8 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
| GSE59703_WhiteselectivePPARgPeaks.bed.gz |
31.8 Kb |
(ftp)(http) |
BED |
| GSE59703_WhiteselectivePPARgSuperenhancers.bed.gz |
4.0 Kb |
(ftp)(http) |
BED |
| GSE59703_Whiteselectivegenes.txt.gz |
100.7 Kb |
(ftp)(http) |
TXT |
| GSE59703_hMADSDHSPeakfile.bed.gz |
373.8 Kb |
(ftp)(http) |
BED |
| GSE59703_hMADSKLF11Peakfile.bed.gz |
494.8 Kb |
(ftp)(http) |
BED |
| GSE59703_hMADSPPARgPeakfile.bed.gz |
801.8 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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