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Series GSE59702 Query DataSets for GSE59702
Status Public on Mar 13, 2015
Title A unifying gene signature for adenoid cystic cancer identifies parallel MYB-dependent and MYB-independent therapeutic targets
Platform organisms Homo sapiens; synthetic construct
Sample organism Homo sapiens
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary MYB activation is proposed to underlie development of adenoid cystic cancer (ACC), an aggressive salivary gland tumor with no effective systemic treatments. To discover druggable targets for ACC, we performed global mRNA/miRNA analyses of 12 ACC with matched normal tissues, and compared these data with 14 mucoepidermoid carcinomas (MEC) and 11 salivary adenocarcinomas (ADC). We detected a unique ACC gene signature of 1160 mRNAs and 22 miRNAs. MYB was the top-scoring gene (18-fold induction), however we observed the same signature in ACC without detectable MYB gene rearrangements. We also found 4 ACC tumors (1 among our 12 cases and 3 from public databases) with negligible MYB expression that retained the same ACC mRNA signature including over-expression of extracellular matrix (ECM) genes. Integration of this signature with somatic mutational analyses suggests that NOTCH1 and RUNX1 participate with MYB to activate ECM elements including the VCAN/HAPLN1 complex. We observed that forced MYB-NFIB expression in human salivary gland cells alters cell morphology and cell adhesion in vitro and depletion of VCAN blocked tumor cell growth of a short-term ACC tumor culture. In summary, we identified a unique ACC signature with parallel MYB-dependent and independent biomarkers and identified VCAN/HAPLN1 complexes as a potential target.
 
Overall design This study included 8 MYB-NFIB fusion positive adenoid cystic cancer (ACC); 4 MYB-NFIB fusion negative adenoid cystic cancer tumors; and 12 matched normal samples respectively. The fusion status was pre-defined using RT-PCR methods. This study includes both miRNA chip and mRNA array expression assays that were performed for the same set of tumors.
 
Citation(s) 25587024
Submission date Jul 23, 2014
Last update date Jul 26, 2018
Contact name Frederic J Kaye
Organization name University of Florida
Street address 2033 Mowry Rd, CGRC365E
City GAINESVILLE
State/province FL
ZIP/Postal code 32603
Country USA
 
Platforms (2)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
GPL8786 [miRNA-1] Affymetrix Multispecies miRNA-1 Array
Samples (48)
GSM1443159 Fusion positive tumor; biological rep1
GSM1443160 Fusion positive tumor; biological rep2
GSM1443161 Match normal of fusion positive tumor; biological rep1
This SuperSeries is composed of the following SubSeries:
GSE59700 A unifying gene signature for adenoid cystic cancer identifies parallel MYB-dependent and MYB-independent therapeutic targets (microRNA)
GSE59701 A unifying gene signature for adenoid cystic cancer identifies parallel MYB-dependent and MYB-independent therapeutic targets (mRNA)
Relations
BioProject PRJNA255980

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59702_RAW.tar 109.5 Mb (http)(custom) TAR (of CEL)

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