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| Status |
Public on Jul 23, 2014 |
| Title |
Temporal Endogenous Gene Expression Profiles in Response to Polymer-Mediated Transfection and Profile Comparison to Lipid-Mediated Transfection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The current understanding of the molecular factors underlying polyethylenimine(PEI)-mediated transfection is largely unknown. Cationic PEI was used to transfer GFP transgene to HEK293T cells. FACS separation of transfected (GFP positive), untransfected (GFP negative), and untreated cells enabled gene expression profiles to be obtained using Affymetrix® HG-U133A 2.0 microarrays for each cell population. Gene profiles were differentially compared for each population combination.
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| Overall design |
Control (untreated), transfected (GFP positive), and untransfected (GFP negative) RNA samples were purified from HEK293T cells after FACS isolation. Treated experiments were repeated on triplicate days to run 3 microarrays for each cell population. Controls were run on duplicate to give 2 microarrays for each population.
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| Contributor(s) |
Martin TM, Plautz S, Pannier AK |
| Citation(s) |
25663627 |
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| Submission date |
Jul 22, 2014 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Timothy Martin |
| Organization name |
University of Nebraska-Lincoln
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| Department |
Biological Systems Engineering
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| Lab |
Pannier
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| Street address |
32 Chase Hall
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| City |
Lincoln |
| State/province |
NE |
| ZIP/Postal code |
68583-0726 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (23)
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| GSM941938 |
HEK293T cell culture, transfected, biological rep 1 |
| GSM941939 |
HEK293T cell culture, transfected, biological rep 2 |
| GSM941940 |
HEK293T cell culture, transfected, biological rep 3 |
| GSM941941 |
HEK293T cell culture, untransfected, biological rep 1 |
| GSM941942 |
HEK293T cell culture, untransfected, biological rep 2 |
| GSM941943 |
HEK293T cell culture, untransfected, biological rep 3 |
| GSM1442091 |
PEI_HEK293T cell culture,2h,untreated,biological rep 1 |
| GSM1442092 |
PEI_HEK293T cell culture,2h,untreated,biological rep 2 |
| GSM1442093 |
PEI_HEK293T cell culture,2h,treated,biological rep 1 |
| GSM1442094 |
PEI_HEK293T cell culture,2h,treated,biological rep 2 |
| GSM1442095 |
PEI_HEK293T cell culture,2h,treated,biological rep 3 |
| GSM1442096 |
PEI_HEK293T cell culture,8h,untransfected,biological rep 1 |
| GSM1442097 |
PEI_HEK293T cell culture,8h,untransfected,biological rep 2 |
| GSM1442098 |
PEI_HEK293T cell culture,8h,untransfected,biological rep 3 |
| GSM1442099 |
PEI_HEK293T cell culture,8h,transfected,biological rep 1 |
| GSM1442100 |
PEI_HEK293T cell culture,8h,transfected,biological rep 2 |
| GSM1442101 |
PEI_HEK293T cell culture,8h,transfected,biological rep 3 |
| GSM1442102 |
PEI_HEK293T cell culture,16h,untransfected,biological rep 1 |
| GSM1442103 |
PEI_HEK293T cell culture,16h,untransfected,biological rep 2 |
| GSM1442104 |
PEI_HEK293T cell culture,16h,untransfected,biological rep 3 |
| GSM1442105 |
PEI_HEK293T cell culture,16h,transfected,biological rep 1 |
| GSM1442106 |
PEI_HEK293T cell culture,16h,transfected,biological rep 2 |
| GSM1442107 |
PEI_HEK293T cell culture,16h,transfected,biological rep 3 |
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| Relations |
| BioProject |
PRJNA255912 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE59665_PEI16h_All_GFP+vGFP-.txt.gz |
2.2 Mb |
(ftp)(http) |
TXT |
| GSE59665_PEI24h_All_GFP+vGFP-.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
| GSE59665_PEI2h_All_GFPvC.txt.gz |
2.5 Mb |
(ftp)(http) |
TXT |
| GSE59665_PEI8h_All_GFP+vGFP-.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
| GSE59665_RAW.tar |
104.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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