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Series GSE59503 Query DataSets for GSE59503
Status Public on Jul 18, 2014
Title miRNA expression profiling and to explore the role of miRNAs in DC120-mediated Sox2 down-regulation
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary From the results of microarray, 7616 miRNAs with significantly different expression ratios were selected when CNE-2-S-18/SP cells were treated with DC120. Among these, 80 miRNAs were up-regulated and 78 were down-regulated. We then chose 20 miRNAs through the qRT – PCR, and our results showed that 9 miRNAs were up-regulated after treatment with DC120, which was consistent with the results of the microarray. Among these, miR-30a were significantly up-regulated. These results indicated that miR 30a expression was up-regulated by DC120. We then investigated the role of miR 30a in DC120-treated cells, we explored its potential targets using a bioinformatics approach of complementary base pairing. The relative luciferase activity of the reporter that contained the wild type 3' UTR was significantly suppressed when miR 30a was contransfected. In contrast, the luciferase activity of the mutant reporter was unaffected by the contransfection of miR 30a, indicating that miR 30a suppressed Sox2 gene expression using the miR 30a binding sequence at the 3' UTR of the Sox2 gene. Additionally, the ectopic expression of miR 30a caused a decrease in Sox2 protein expression and mRNA levels in CNE-2-S-18 cells. These data demonstrate that miR 30a may directly target the Sox2 gene via seed matches on both 3' UTR. Taken together, our results demonstrate that DC120 regulates Sox2 via up-regulation of miR 30a.
 
Overall design In this study, two samples were used to acquire the miRNA expression profiling. We analyzed the differences in miRNA profiles between control (DMSO solution) and DC120 treatment in CNE-2-S-18/SP cells from miRNA microarrays. The CNE-2-S-18/SP cells were separately cultured with a DMSO solution or 10μmol/L DC120 for 15h, and total RNA from all of the cells was extracted. Then we chose 20 up-regulated miRNAs through the qRT – PCR, and our results showed that 9 miRNAs were up-regulated after treatment with DC120. Among these, miR-30a were significantly up-regulated. Then we investigated the role of miR 30a in DC120-treated cells by bioinformatic methods and Dual luciferase reporter system, the function of miR-30a was analyzed through ectopic expression of miR 30a. Taken together, our results demonstrate that DC120 regulates Sox2 via up-regulation of miR 30a.
 
Contributor(s) Qin J, Ji J
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Submission date Jul 17, 2014
Last update date May 24, 2017
Contact name jiao ji
E-mail jijiao@sysucc.org.cn
Phone 18565233766
Organization name Sun Yat-Sen University Cancer Center
Department State Key Laboratory of Oncology in Southern China
Street address 651 Dongfeng Road East
City Guangzhou
State/province Guangdong
ZIP/Postal code 510060
Country China
 
Platforms (1)
GPL18116 Exiqon miRCURY LNA microRNA array, 7th generation REV [miRBase v18.0, condensed Probe_ID version]
Samples (2)
GSM1438490 S18/SP_0_15h_rep1
GSM1438491 S18/SP_10uM_15h_rep1
Relations
BioProject PRJNA255466

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Supplementary file Size Download File type/resource
GSE59503_RAW.tar 1.9 Mb (http)(custom) TAR (of GPR)
Raw data provided as supplementary file
Processed data included within Sample table

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