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Series GSE59451 Query DataSets for GSE59451
Status Public on Nov 11, 2014
Title The ω subunit of RNA polymerase is essential for thermal acclimation of the Cyanobacterium Synechocystis sp. PCC 6803
Organism Synechocystis sp. PCC 6803
Experiment type Expression profiling by array
Summary The rpoZ gene encodes the small ω subunit of RNA polymerase (RNAP). A ∆rpoZ strain of the cyanobacterium Synechocystis sp. PCC 6803 grew well in standard conditions (constant illumination at 40 µmol photons m-2s-1; 32 °C; ambient CO2) but was heat sensitive and died at 40 °C. In the control strain , 71 genes were at least two-fold up-regulated and 91 genes down-regulated after a 24-h treatment at 40 °C, while in ∆rpoZ 394 genes responded to heat. Only 62 of these heat-responsive genes were similarly regulated in both strains, and 80 % of heat-responsive genes were unique for ΔrpoZ. The RNAP core and the primary σ factor SigA were down-regulated in control strain at 40 °C, but not in ΔrpoZ. In accordance with reduced RNAP content, the total RNA content of mild-heat-stress-treated cells was lower in control strain than in ΔrpoZ. Light-saturated photosynthetic activity decreased more in ΔrpoZ than in control strain upon mild heat stress. The amounts of Photosystem II and Rubisco decreased at 40 °C in both strains while PSI and the phycobilisome antenna protein allophycocyanin remained at the same level as in standard conditions. The phycobilisome rod proteins, phycocyanins, diminished during the heat treatment in ΔrpoZ but not in control strain, and the nblA1 and nblA2 genes (encode NblA proteins required for phycobilisome degradation) were up-regulated only in ΔrpoZ. Our results show that the ω subunit of RNAP is essential in heat stress because it is required for heat acclimation of diverse cellular processes.
Overall design Cells from cyanobacteria Synechocystis sp. PCC 6803 named as control strain (CS) and RNA polymerase omega subunit inactivation strain, ΔrpoZ, were harvested (A730=1, 40 mL) after treated at 40 °C under continuous illumination, PPFD 40 µmol m-2s-1, for 24 h.
Contributor(s) Tyystjarvi T, Gunnelius L, Hakkila K, Matthijs HC
Citation(s) 25386944
Submission date Jul 16, 2014
Last update date Feb 16, 2015
Contact name Taina Tyystjarvi
Organization name University of Turku
Department Biochemistry
Lab Molecular Plant Biology
Street address Pharmacity/Itäinen Pitkäkatu 4 C, 6th floor
City Turku
ZIP/Postal code 20520
Country Finland
Platforms (1)
GPL17595 Agilent-016989 Synechocystis array [ORF version]
Samples (13)
GSM1250061 CS_standard_biol.rep1 (reanalysis)
GSM1250062 CS_standard_biol.rep2 (reanalysis)
GSM1250063 CS_standard_biol.rep3 (reanalysis)
BioProject PRJNA255362

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59451_RAW.tar 9.8 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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