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Series GSE59371 Query DataSets for GSE59371
Status Public on Mar 30, 2015
Title Deletion of the gene family of small chlorophyll-binding proteins (ScpABCDE) offsets C/N homeostasis in synechocystis PCC 6803
Organism Synechocystis sp. PCC 6803
Experiment type Expression profiling by array
Summary Small CAB-like proteins (SCPs) are single-helix light-harvesting-like proteins found in all organisms performing oxygenic photosynthesis. We investigated the function of these stress-induced proteins in the cyanobacterium Synechocystis sp. PCC 6803 by comparing a strain, which expresses SCPs constitutively, with a mutant lacking all five SCPs. In the absence of SCPs, cells were larger, and showed irregular thylakoid structure and cell-surfaces. Deletion of scp genes strongly affected the carbon-nitrogen balance, causing accumulation of carbohydrates and a decrease in N-rich compounds (proteins and chlorophyll a). Data from transcriptomic and metabolomic experiments demonstrated that SCPs mediated stabilization of chlorophyll a under stress conditions is crucial to maintain the C/N balance. SCPs diminished the formation of reactive oxygen species, preventing cellular damage by adjusting the de-novo production of chlorophyll a to demand levels. Lack of SCP expression under stress conditions had a large impact on the metabolism of the entire cell.
 
Overall design Synechocystis 6803 PSI-less (Shen et al 1993) and PSI-less/ScpABCDE- (Xu et al 2004) mutants were cultivated at 30°C at a light intensity of 4–5 μmol photons m–2 s–1 in BG-11 growth medium (Rippka et al 1979) supplemented with 10 mM glucose. Cells (40–50 ml) from both control and the mutant cultures were collected at mid-log phase by rapid filtration (Pall Supor 800 Filter, 0.8 mm) and total RNA extracted after Pinto et al. (Pinto et al 2009). Single-stranded cDNA preparation, labelling, and hybridization onto the microarray slides were performed after Eisenhut et al. (2007). The microarray slides were scanned with an Agilent Microarray Scanner (model G2505B) with default settings.
Web link http://www.sciencedirect.com/science/article/pii/S0005272815002431
 
Contributor(s) Hernández-Prieto MA
Citation(s) 26646103
Submission date Jul 14, 2014
Last update date Jan 04, 2016
Contact name Miguel A Hernández-Prieto
Organization name University of Sydney
Department School of life and environmental sciences
Lab ARC centre of excellence for translational photosynthesis
Street address Macleay Building A12
City Sydney
State/province NSW
ZIP/Postal code 2001
Country Australia
 
Platforms (1)
GPL17595 Agilent-016989 Synechocystis array [ORF version]
Samples (3)
GSM1435995 PSI-less/Scp-less, Biological replicate 1 of 3
GSM1435996 PSI-less/Scp-less, Biological replicate 2 of 3
GSM1435997 PSI-less/Scp-less, Biological replicate 3 of 3
Relations
BioProject PRJNA255208

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59371_RAW.tar 3.0 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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