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Series GSE59252 Query DataSets for GSE59252
Status Public on Mar 26, 2015
Title Functional diversity and convergence of small non-coding RNAs in male germ cell differentiation and fertilization (mRNA analysis)
Organism Mus musculus
Experiment type Expression profiling by array
Summary The small non-coding RNAs (sncRNAs) are considered as postranscriptional key regulators of male germ cell development. In addition to microRNAs (miRNAs) and PIWI-interacting RNAs (piRNAs), other sncRNAs generated from small nucleolar RNAs (snoRNAs), tRNAs or rRNAs processing may also play important regulatory roles in spermatogenesis. By next generation sequencing (NGS), we characterized the different sncRNA populations detected at three milestone stages in male germ differentiation: primordial germ cells (PGCs) at 13.5 dpc, pubertal spermatogonia cells, and mature spermatozoa. In order to assess the potential transmission of the sncRNAs through the mature spermatozoa during fertilization, the sncRNA population detected in male germ cells was also compared with sncRNAs detected in unfertilized mouse oocytes and zygotes. Combining the data obtained by NGS and microarrays from whole PGC and spermatogonia transcriptome, we defined the potential regulatory roles of specific miRNAs and their validated targets. Similar to miRNAs, both the small RNAs derived from snoRNAs and the piRNAs, could be involved in the postranscriptional regulation of mRNA transcripts during the male germ development. Finally, our results strongly suggest that the small RNAs-derived from tRNAs and rRNAs are interacting with PIWI proteins, and specifically with MILI. These new classes of piRNAs are not generated by the ping-pong pathway and could be the source of primary piRNAs.
Overall design mRNA analysis of Primordial Germ Cells (PGCs), Spermatogonia cells (SPG), adult testis (AdT) and Gonad-less (GL) embryos. Indirect comparisons were made across multiple arrays with raw data pulled from different channels for data analysis and comparison to the control data.
Contributor(s) García-López J, Cárdenas D, Alonso L, Artaza H, Hourcade JD, Martínez S, Brieño MA, del Mazo J
Citation(s) 25805854
Submission date Jul 09, 2014
Last update date Mar 27, 2015
Contact name Jesus Garcia Lopez
Phone +34 918373112
Organization name Cib (CSIC)
Department Cellular and Molecular Biology
Lab Molecular biology of gametogenesis
Street address C/Ramiro de Maeztu 9
City Madrid
ZIP/Postal code 28040
Country Spain
Platforms (1)
GPL18911 MI Ready Array Mouse 49K (MEEBO)
Samples (3)
GSM1431882 PGCs gene expression
GSM1431883 Spermatogonia cells gene expression
GSM1431884 Adult testis gene expression
This SubSeries is part of SuperSeries:
GSE59255 Functional diversity and convergence of small non-coding RNAs in male germ cell differentiation and fertilization
BioProject PRJNA254812

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59252_RAW.tar 18.5 Mb (http)(custom) TAR (of GPR)
GSE59252_README.txt 147 b (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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