|
| Status |
Public on Sep 30, 2015 |
| Title |
Single cell RNA_Seq in aging of hematopoietic stem and progenitor cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here, we use single-cell RNA-Seq to examine variation between individual hematopoietic stem and progenitor cells from two mouse strains (C57BL/6 and DBA/2) as they age. We prepared libraries from long-term (LT-HSCs) (LSK CD150+CD48-), short-term hematopoietic stem cells (ST-HSCs) (LSK CD150-CD48-) and multipotent progenitors (MPPs) (LSK CD150+CD48+) from young (2-3 months) and old mice (22 months for C57BL/6 and 20 months for DBA/2). Population controls for each cell type and age were isolated by sorting processed in parallel.
|
| |
|
| Overall design |
RNA seq libraries from single cells and population controls. All prepared with SMARTer protocol for cDNA and amplification followed by Nextera protocol for library preparation.
|
| |
|
| Contributor(s) |
Kowalczyk MS |
| Citation(s) |
26430063 |
| |
| Submission date |
Jul 07, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Monika Sylwia Kowalczyk |
| Organization name |
Broad Institute
|
| Lab |
Regev Lab
|
| Street address |
415 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (2128)
|
|
| Relations |
| BioProject |
PRJNA254994 |
| SRA |
SRP044256 |
Less...
More...