|
| Status |
Public on Oct 07, 2014 |
| Title |
ETS1 is a genome-wide effector of RAS/ERK signaling in epithelial cells (RNA-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
ETS1 and RAS/ERK regulate a common gene expression program in establishing enviroment suitable for prostate cancer cell migration.
|
| |
|
| Overall design |
mRNA profiles of luciferase knockdown (WT), ETS1 knockdown, and U0126 treated DU145 cells were generated using deep sequencing, in triplicate, using Illumina HiSeq. Knockdowns were stable shRNA expression from a lentiviral construct selected with puromycin.
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| |
|
| Contributor(s) |
Plotnik JP, Budka JA, Hollenhorst PC |
| Citation(s) |
25294825, 28119415 |
| |
| Submission date |
Jul 02, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Josh Plotnik |
| E-mail(s) |
joshua.plotnik@abbvie.com
|
| Organization name |
AbbVie Inc.
|
| Street address |
1 North Waukegan Rd, AP10/210A
|
| City |
North Chicago |
| State/province |
IL |
| ZIP/Postal code |
60064 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (9)
|
| GSM1424515 |
Luciferase knockdown in DU145 cells using shRNA replicate 1 |
| GSM1424516 |
Luciferase knockdown in DU145 cells using shRNA replicate 2 |
| GSM1424517 |
Luciferase knockdown in DU145 cells using shRNA replicate 3 |
| GSM1424518 |
ETS1 knockdown in DU145 cells using shRNA replicate 1 |
| GSM1424519 |
ETS1 knockdown in DU145 cells using shRNA replicate 2 |
| GSM1424520 |
ETS1 knockdown in DU145 cells using shRNA replicate 3 |
| GSM1424521 |
U0126 treated DU145 cells replicate 1 |
| GSM1424522 |
U0126 treated DU145 cells replicate 2 |
| GSM1424523 |
U0126 treated DU145 cells replicate 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE59022 |
ETS1 is a genome-wide effector of RAS/ERK signaling in epithelial cells |
|
| Relations |
| BioProject |
PRJNA254145 |
| SRA |
SRP044013 |
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