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Series GSE59001 Query DataSets for GSE59001
Status Public on May 18, 2015
Title BIM is the primary mediator of MYC-induced apoptosis in multiple solid tissues
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary C-MYC (henceforth MYC) is one of the most frequently overexpressed oncogenes in human cancer and even modestly deregulated MYC expression can initiate ectopic proliferation in many post-mitotic, terminally differentiated cell types in vivo. Metazoan organisms have consequently evolved a number of mechanisms to counteract MYC's oncogenic potential, of which apoptosis is arguably the best understood. However, the mechanisms through which MYC induces apoptosis remains controversial, with some studies implicating p19ARF-mediated stabilization of p53, followed by induction of pro-apoptotic BH3 family member NOXA and PUMA, while others argue for more direct regulation of BH3 proteins, especially BIM. The debate likely stems from the use of different experimental systems, modes of perturbation, and quite possibly different levels of MYC expression. Here, we use a single experimental system to systematically evaluate the roles of p19ARF and BIM during MYC-induced apoptosis, in vitro, in vivo, and in combination with a widely used tumoricidal chemotherapeutic, Doxorubicin. We find a common specific requirement for BIM during MYC-induced apoptosis in multiple settings, which does not extend to the p53-responsive BH3 family member PUMA, and find no evidence of a role for p19ARF during MYC-induced apoptosis in the tissues examined.
 
Overall design MYC-ER ChIP-Seq with HC20 anti-ER antibody in MCF10A cells performed on an Illumina IIx Genome Analyzer.
 
Contributor(s) Muthalagu N, Juntilla M, Wiese KE, Wolf E, Bauer B, Morton J, Evan GI, Eilers M, Murphy DJ
Citation(s) 25176652
Submission date Jul 01, 2014
Last update date May 15, 2019
Contact name Martin Eilers
Organization name University of Wuerzburg
Department Chair for Biochemistry and Molecular Biology
Lab Martin Eilers
Street address Am Hubland
City Wuerzburg
ZIP/Postal code 97074
Country Germany
 
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (2)
GSM1423725 MCF10A_HC20_MYC-ER_ChIP-Seq
GSM1423726 MCF10A_Input_ChIP-Seq
Relations
BioProject PRJNA254075
SRA SRP043969

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Supplementary file Size Download File type/resource
GSE59001_RAW.tar 322.4 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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