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Series GSE58998 Query DataSets for GSE58998
Status Public on Feb 09, 2015
Title The chromatin-modifying enzyme Ezh2 is critical for the maintenance of regulatory T cell identity after activation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Regulatory T cells (Tregs) are essential for maintaining proper immune homeostasis. Extracellular signals (e.g. TCR, CD28, IL-2R) are necessary for the generation and maintenance of Tregs, but how these signals are integrated to control the gene expression patterns of Tregs is less clear. Here we show that the epigenetic regulator, Ezh2, was induced by CD28 costimulation and Ezh2 activity was elevated in Tregs as compared to conventional CD4+ T cells. Deletion of Ezh2 in mouse Tregs led to a progressive autoimmune disease because Tregs were compromised after activation, losing proper control of essential Treg lineage genes and adopting a gene expression pattern similar to Foxp3-deficient ‘Tregs.’ Lineage-tracing of Ezh2-deficient Tregs in vivo confirmed that the cells were destabilized selectively in activated Treg populations, which led to a significant loss of Tregs in non-lymphoid tissues. These studies reveal an essential role for Ezh2 in the maintenance of Treg “identity” during cellular activation and differentiation.
 
Overall design RNAseq of sorted populations of CD62Lhi or CD62Llo Tregs for both Ezh2-HET (Foxp3YFP-Cre/Foxp3WT;Ezh2fl/+ female mice) and Ezh2-KO (Foxp3YFP-Cre/Foxp3WT;Ezh2fl/fl female mice) were generated, in triplicate for each condition, using Illumina HiSeq 2500 single-end 50bp sequencing platform.
 
Contributor(s) Chopra G, DuPage M, Bluestone JA
Citation(s) 25680271
Submission date Jul 01, 2014
Last update date May 15, 2019
Contact name GAURAV CHOPRA
E-mail(s) gchopra@diabetes.ucsf.edu
Organization name UNIVERSITY OF CALIFORNIA - SAN FRANCISCO (UCSF)
Department DIABETES CENTER
Lab BLUESTONE LAB
Street address 513 PARNASSUS AVE, HSW 1102
City SAN FRANCISCO
State/province CA
ZIP/Postal code 94109
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM1423625 Ezh2-HET-CD62Lhi rep1
GSM1423626 Ezh2-HET-CD62Lhi rep2
GSM1423627 Ezh2-HET-CD62Lhi rep3
Relations
BioProject PRJNA254138
SRA SRP044008

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58998_FPKM_all_samples_merged.txt.gz 1.3 Mb (ftp)(http) TXT
GSE58998_RAW.tar 8.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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