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Series GSE5883 Query DataSets for GSE5883
Status Public on May 29, 2009
Title Lipopolysaccharide-induced Changes in Gene Expression in Human Lung Microvascular Endothelial Cells over Time
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The vascular endothelium may play a role in the response to infectious agents and in the pathophysiology of disease processes resulting in capillary leak such as septic shock and acute respiratory distress syndrome. In order to study the effect of endotoxin on endothelial cell function, human lung microvascular endothelial cells in culture were exposed to lipopolysaccharide (LPS), 10 ng, for 4, 8, or 24 hours and changes in mRNA expression were studied using Affymetrix HG U133plus2 gene arrays. A principal components analysis revealed that LPS treatment was the primary source of variability in the data. LPS treatment of endothelial cells for 4, 8, or 24 hours resulted in the upregulation by two-fold or greater of 275, 260 and 141 genes respectively. LPS treatment resulted in the down regulation by 50% or greater of 176, 263 and 79 genes at 4, 8, or 24 hours respectively. Up regulated genes at 4 or 8 hours were enriched in those encoding for cytokines, secreted proteins, cell membrane proteins and proteins controlling signal transduction and transcriptional regulation. Down regulated genes at each of the time points included those coding for cell membrane proteins, transcriptional regulation and metabolism. At each time point, a significant proportion of the genes identified as changed were unique to that time point.
Keywords: Time course
 
Overall design In order to study the effect of endotoxin on endothelial cell function, human lung microvascular endothelial cells in culture were exposed to lipopolysaccharide (LPS), 10 ng, for 4, 8, or 24 hours and changes in mRNA expression were studied using Affymetrix HG U133plus2 gene arrays. Controls for each time point with no LPS exposure were also run. An N of 4 for each time point and treatment was used.
 
Contributor(s) Harry AC, Joubert D, Munson PJ, Barb J, Woszczek G, Nagineni S, Logun C, Suffredini AF, Danner RL, Shelhamer JH
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Submission date Sep 20, 2006
Last update date Jan 08, 2019
Contact name Anya Carmen Harry
E-mail aharry@cc.nih.gov
Phone 301-496-9320
Fax 301-402-1213
Organization name NIH
Department CCMD
Street address 10 Center Dr. MSC 1662 Bldg 10
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (24)
GSM114552 HMVEC-L stimulated with LPS for 4 hours L04R1
GSM114553 HMVEC-L stimulated with LPS for 4 hours L04R2
GSM114554 HMVEC-L stimulated with LPS for 4 hours L04R3
Relations
BioProject PRJNA97301

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