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Series GSE58491 Query DataSets for GSE58491
Status Public on Dec 23, 2014
Title Characterizing the profiles of histone markers in mouse adipocytes during insulin resistance
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Insulin resistance is a sine qua non of Type 2 diabetes (T2D) and a frequent complication of multiple clinical conditions, including obesity, aging, and steroid use, among others. How such a panoply of insults can result in a common phenotype is incompletely understood. Furthermore, very little is known about the transcriptional and epigenetic basis of this disorder, despite evidence that such pathways are likely to play a fundamental role. Here, we compare cell autonomous models of insulin resistance induced by the cytokine tumor necrosis factor-a (TNF) or by the steroid dexamethasone (Dex) to construct detailed epigenomic maps associated with cellular insulin resistance.
Overall design Murine 3T3-L1 adipocytes were treated separately with dexamethasone (Dex; 20nM) or tumor necrosis factor-alpha. To comprehensively assess epigenomic changes caused by Dex and TNF in a time-dependent manner, we profiled cells at early (2 hours), intermediate (24 hours), and late (6 days) points in the development of insulin resistance.
Contributor(s) Kang S, Tsai L, Zhou Y, Rosen ED
Citation(s) 25503565
Submission date Jun 13, 2014
Last update date May 15, 2019
Contact name Evan Rosen
Organization name Beth Israel Deconess Medical Center
Department Endocrinology
Lab Rosen Lab
Street address 3 Blackfan Cir
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (66)
GSM1412455 Dex24H_H3K27ac
GSM1412456 Dex24H_H3K27me3
GSM1412457 Dex24H_H3K36me3
BioProject PRJNA252738
SRA SRP043216

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Supplementary file Size Download File type/resource
GSE58491_RAW.tar 36.0 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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