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| Status |
Public on Dec 23, 2014 |
| Title |
Characterizing the profiles of histone markers in mouse adipocytes during insulin resistance |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Insulin resistance is a sine qua non of Type 2 diabetes (T2D) and a frequent complication of multiple clinical conditions, including obesity, aging, and steroid use, among others. How such a panoply of insults can result in a common phenotype is incompletely understood. Furthermore, very little is known about the transcriptional and epigenetic basis of this disorder, despite evidence that such pathways are likely to play a fundamental role. Here, we compare cell autonomous models of insulin resistance induced by the cytokine tumor necrosis factor-a (TNF) or by the steroid dexamethasone (Dex) to construct detailed epigenomic maps associated with cellular insulin resistance.
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| Overall design |
Murine 3T3-L1 adipocytes were treated separately with dexamethasone (Dex; 20nM) or tumor necrosis factor-alpha. To comprehensively assess epigenomic changes caused by Dex and TNF in a time-dependent manner, we profiled cells at early (2 hours), intermediate (24 hours), and late (6 days) points in the development of insulin resistance.
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| Contributor(s) |
Kang S, Tsai L, Zhou Y, Rosen ED |
| Citation(s) |
25503565 |
| Submission date |
Jun 13, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Evan Rosen |
| E-mail(s) |
erosen@bidmc.harvard.edu
|
| Organization name |
Beth Israel Deconess Medical Center
|
| Department |
Endocrinology
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| Lab |
Rosen Lab
|
| Street address |
3 Blackfan Cir
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (66)
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| Relations |
| BioProject |
PRJNA252738 |
| SRA |
SRP043216 |
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