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Series GSE58442 Query DataSets for GSE58442
Status Public on Sep 23, 2014
Title Differential gene expression in the skin of dogs sensitized to the house dust mite Dermatophagoides farinae
Organism Canis lupus familiaris
Experiment type Expression profiling by array
Summary Atopic dermatitis is a multifactorial allergic skin disease in humans and dogs. Genetic predisposition, immunologic hyperreactivity, a defective skin barrier and environmental factors play a role in its pathogenesis. The aim of this study was to analyze gene expression in the skin of dogs sensitized to house dust mite antigens. Skin biopsies were collected from six sensitized and six non-sensitized Beagle dogs from normal, non-treated skin before and six and 24 hours after challenge using skin patches with allergen or saline as a negative control. Transcriptome analysis was performed by the use of DNA microarrays and expression of selected genes was validated by quantitative real-time RT-PCR. Expression data was compared between groups (unpaired design). After 24 hours 597 differentially expressed genes were detected, 361 with higher and 226 with lower mRNA concentration in allergen treated skin of sensitized dogs compared to their saline-treated skin and compared to the control specimens. Functional annotation clustering, pathway-and co-citation analysis showed, that the genes with increased expression were involved in inflammation, wound healing and immune response. In contrast, genes with decreased expression in sensitized dogs were associated with differentiation and barrier function of the skin. As the sensitized dogs did not show differences in the untreated skin compared to controls, inflammation after allergen patch test probably led to a decrease in the expression of genes important for barrier formation. Our results further confirm the similar pathophysiology of human and canine atopic dermatitis and revealed genes previously not known to be involved in canine atopic dermatitis.
Overall design 60 canine (dog) skin tissue samples; six sensitized and six non-sensitized Beagle dogs; samples collected before (0h), 6h and 24h after challenge with allergen; samples collected from a skin area treated with saline and from an area treated with allergen
Contributor(s) Schamber P, Schwab-Richards R, Müller R, Bauersachs S
Citation(s) 25098772
Submission date Jun 12, 2014
Last update date Sep 24, 2014
Contact name Stefan Michael Bauersachs
Organization name University of Zurich
Department Department for Farm Animals
Lab Genetics and Functional Genomics
Street address Eschikon 27 EHB 23.1
City Lindau
State/province Zurich
ZIP/Postal code 8315
Country Switzerland
Platforms (1)
GPL18789 Agilent-031538 Canis familiaris Cfa_custom_20101122 021193
Samples (60)
GSM1411137 A_2838_0h
GSM1411138 A_2838_6h_A
GSM1411139 A_2838_6h_S
BioProject PRJNA252617

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Supplementary file Size Download File type/resource
GSE58442_RAW.tar 187.6 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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