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Series GSE58300 Query DataSets for GSE58300
Status Public on Sep 25, 2014
Title Gene expression profiles of mouse ESC cardiomyocyte-specific differentiation
Organism Mus musculus
Experiment type Expression profiling by array
Summary The differentiation to cardiomyocytes is a prerequisite and an important part of heart development. A good understanding of the complicated cardiomyocyte differentiation process benefits cardiogenesis study. Embryonic stem cells (ESCs), cell lines with infinite ability to proliferate and to be differentiated into all cell types of the adult body, are important research tools for investigation of differentiation and meanwhile good models for developmental research. In the current study, genome-wide gene expression of ESCs is profiled through high throughput platform during cardiomyocyte-specific differentiation and maturation. Gene expression patterns of undifferentiated ESCs and ESC-derived cardiomyocytes provide a global overview of genes involved in cardiomyocyte-specific differentiation, whereas marker gene expression profiles of both ESC-related genes and cardiac-specific genes presented the expression pattern shift during differentiation in a pure ESC-derived cardiomyocyte cell culture system.
Overall design Transgenic mouse ESC clone with α-MHC–Pac–IRES–EGFP vector containing the EGFP gene and the PuromycinR (Pac) cassette under control of the cardiac α-myosin heavy chain (α-MHC) promoter was cultured and induced into differentiation. Puromycin was applied after differentiation start to enable cardiomyocyte-specific differentiation. Cells were harvested at 4 time points after differentiation start (day0, day12, day19 and day26). Two biological replicates were taken for every time point.
Contributor(s) Gan L, Schwengberg S, Denecke B
Citation(s) 25058891
Submission date Jun 09, 2014
Last update date Mar 06, 2018
Contact name Lin Gan
Organization name University Hospital Aachen
Department IZKF
Lab Chip facility
Street address Pauwelsstr. 30
City Aachen
ZIP/Postal code 52074
Country Germany
Platforms (1)
GPL6096 [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version]
Samples (8)
GSM1406222 mESC_day0_rep1
GSM1406223 mESC_day0_rep2
GSM1406224 mESC_day12_rep1
BioProject PRJNA251956

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58300_Mm_Ensembl_probesets.txt.gz 15.9 Mb (ftp)(http) TXT
GSE58300_RAW.tar 190.8 Mb (http)(custom) TAR (of CEL)
GSE58300_matrix_RMA_normalized.txt.gz 1.1 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data are available on Series record

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