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| Status |
Public on Dec 18, 2014 |
| Title |
Whole-genome GR binding sites and histone acetylation status in pediatric acute lymphoblastic leukemia patient-derived xenografts following dexamethasone treatment in vivo |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Glucocorticoids are critical components of combination chemotherapy regimens in pediatric acute lymphoblastic leukemia (ALL). However, the signaling pathways regulating apoptosis in glucocorticoid-treated lymphoid cells remain unclear. In this study, pediatric ALL patient-derived xenograft inherently sensitive to glucocorticoids were exposed to dexamethasone in vivo. Whole-genome GR binding sites and histone acetylation status were detected using chromatin immunoprecipitation sequencing analyses. This provided a global understanding of dexamethasone-induced DNA modulations in ALL cells in vivo, which is likely to be important in the understanding of mechanisms of glucocorticoid response in lymphoid malignancies.
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| Overall design |
One xenograft (ALL-54) was derived from a patient of dexamethasone-good responder. The xenograft was innoculated into 2 NOD/SCID mice, and treated with dexamethasone (15 mg/kg) or vehicle control. Binding of glucocorticoid receptor (GR), histone acetylation and IgG control in spleen-harvest xenograft samples were detected using ChIP-seq.
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| |
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| Contributor(s) |
Jing D, Beck D, Wong JW, Pimanda JE, Lock RB |
| Citation(s) |
25336632 |
| |
| Submission date |
Jun 06, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Dominik Beck |
| E-mail(s) |
dominik.beck@uts.edu.au
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| Organization name |
University of Technology Sydney
|
| Department |
School of Biomedical Engineering
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| Street address |
Level 10, UTS Building 11 (CB11 81-113, Broadway, Ultimo)
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2007 |
| Country |
Australia |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA251815 |
| SRA |
SRP042995 |
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