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Series GSE58184 Query DataSets for GSE58184
Status Public on Jun 20, 2014
Title The role of the transcription factors LacR and LacT in the regulation of the lactose and galactose utilization pathways in Streptococcus pneumoniae
Organism Streptococcus pneumoniae D39
Experiment type Expression profiling by array
Summary Transcriptome comparison of the Streptococcus pneumoniae strain D39 grown in the presence of either lactose or galactose with that of the strain grown in the presence of glucose, revealed the elevated expression of various genes and operons, including the lac gene cluster that is organized into two operons i.e. lac operon-I (lacABCD) and lac operon-II (lacTFEG). Deletion of the DeoR family transcriptional regulator lacR that is present downstream of the lac gene cluster, revealed elevated expression of lac operon-I, even in the absence of lactose. This suggests a function of LacR as a transcriptional repressor of lac operon-I that encodes enzymes involved in the Tagatose-6-P pathway in the absence of lactose or galactose. Deletion of lacR did not affect the expression of lac operon-II that encodes for a lactose-specific PTS. This finding was further confirmed by ?-galactosidase assays with PlacA-lacZ and PlacT-lacZ in the presence of either lactose or glucose as a sole carbon source in the medium. This suggests the presence of another transcriptional regulator in the regulation of lac operon-II, which could be the BglG-family transcriptional antiterminator LacT. We demonstrate the role of LacT as a transcriptional activator of lac operon-II in the presence of lactose and CcpA-independent regulation of the lac gene cluster in S. pneumoniae.

This SuperSeries is composed of the SubSeries listed below.
Overall design Refer to individual Series
Citation(s) 24951784
Submission date Jun 03, 2014
Last update date Nov 02, 2016
Contact name Sulman Shafeeq
Organization name Karolinska Institutet
Department MTC
Street address nobel vag 16
ZIP/Postal code 17177
Country Sweden
Platforms (1)
GPL11484 Streptococcus pneumoniae D39 Multiplatform 1909k array
Samples (9)
GSM1402810 Slide 344293
GSM1402811 Slide 344294
GSM1402812 Slide 343739
This SuperSeries is composed of the following SubSeries:
GSE58180 Transcriptional response of Streptococcus pneumoniae D39 to Galactose
GSE58181 Transcriptional response of Streptococcus pneumoniae D39 to Lactose
GSE58182 Impact of lacT delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Lactose
BioProject PRJNA251520

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Supplementary file Size Download File type/resource
GSE58184_RAW.tar 4.8 Mb (http)(custom) TAR (of TXT)
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