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| Status |
Public on Dec 31, 2014 |
| Title |
Evaluation of MicroRNA Expression Profiles for Form-Deprivation Myopia in Mouse |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
The study aim was to determine microRNA (miRNA) expression profiles of ocular tissues in form-deprivation induced myopia (FDM) mice. Form-deprivation myopia was induced in C57BL/6Jmice over the right eye; the contralateral left eyes were used as controls. Whole genome microRNA expression profiles in myopic whole eye, retina, and sclera were determined using the Agilent mouse miRNA microarray. The normalized microarray data were performed ANOVA test to identify differences in miRNA expression between myopic and control eyes. The differential expression for selected miRNAs was validated by quantitative real time PCR (qRT-PCR).
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| Overall design |
Fifteen C57BL/6J mice were used in this study. Form-deprivation myopia was induced over the right eyes; the contralateral left eyes were used as controls. Total RNA was isolated from the whole eye (6 mice), retina (9 mice), and sclera (9 mice) and was used for miRNA expression profiling with the Agilent Mouse miRNA v.15 Microarray.
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| Contributor(s) |
Luo X, Tkatchenko TV, Tkatchenko A, Gonzalez P, Young TL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 30, 2014 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Terri Young |
| Organization name |
Duke University
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| Department |
Ophthalmology
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| Street address |
LaSale St.
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL15880 |
Agilent-029152 Unrestricted Mouse miRNA v15.0 Microarray 028772 (Probe Name version) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA249066 |
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