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Series GSE57898 Query DataSets for GSE57898
Status Public on Jan 01, 2015
Title Transcriptomic analysis of APC knockdown in proliferating primary myoblasts
Organism Mus musculus
Experiment type Expression profiling by array
Summary APC is a key regulator of canonical Wnt signalling since it participates to beta-catenin targeting to proteasomal degradation when the pathway is inactive. Moreover, independently of Wnt signaling, APC regulates several cellular functions such as mycrotubule dynamics, chromosome segregation, cell adhesion. Although APC has been widely studied for its implication in initation and progression of several cancers, its role in satellite cells (skeletal muscle stem cells) has never been investigated.
Here we used microarrays and to clarify APC functions and we identified several pathways and cellular processes to be affected following APC silencing.
Overall design We cultured satellite cells-derived primary myoblasts in proliferating conditions and we transfected them either by a siRNA targeting APC or by a control siRNA. 48 hours after transfection, cells were processed for RNA extraction.
Contributor(s) Parisi A, Le Grand F
Citation(s) 26304725
Submission date May 22, 2014
Last update date Aug 28, 2015
Contact name Fabien Le Grand
Phone 0033144412436
Organization name Institut Cochin
Department DRC
Street address 24 rue du Fg Saint Jacques
City Paris
ZIP/Postal code 75014
Country France
Platforms (1)
GPL17777 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: mogene10st_Mm_ENTREZG_17.1.0]
Samples (6)
GSM1397204 myoblasts-control siRNA-biological repl 01
GSM1397205 myoblasts-control siRNA-biological repl 02
GSM1397206 myoblasts-control siRNA-biological repl 03
BioProject PRJNA248381

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Supplementary file Size Download File type/resource
GSE57898_RAW.tar 48.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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