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Series GSE57854 Query DataSets for GSE57854
Status Public on Jan 12, 2015
Title Retinoic acid induces Snai1 in stem cells of the preimplantation blastocyst to initiate differentiation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Although much is known about the pluripotency self-renewal circuitry, the molecular events that lead embryonic stem cells (ESCs) exit from pluripotency and begin differentiation are largely unknown. We found that the zinc finger transcription factor Snai1, involved in gastrulation and epithelial- mesenchymal transition (EMT) is already expressed in the inner cell mass of the preimplantation blastocysts. In ESCs Snai1 does not respond to TGFα or BMP4 signalling but it is induced by retinoic acid (RA) treatment, which induces the binding, on the Snai1 promoter, of the retinoid receptors RARγ and RXRα the dissociation of the Polycomb repressor compex 2 (PRC2) which results in the decrease of H3K27me3 and the increase of histone H3K4me3. Snai1 mediates the repression of pluripotency genes by binding directly to the promoters of Nanog, Nr5a2, Tcl1, c-Kit, and Tcfcp2l1. The transient activation of Snai1 in embryoid bodies induces the expression of the markers of all three germ layers. These results suggest that Snai1 is a key factor that triggers ESCs exit from the pluripotency state and initiate their differentiation processes.
Overall design microarray analysis of embryonic stem cells (ESC) expressing Snail-ER at various time points of induction with 4-OHT
Web link
Contributor(s) Neri F, Oliviero S, Galvagni F
Citation(s) 25504116, 26484184
Submission date May 21, 2014
Last update date Jan 16, 2019
Contact name Francesco Neri
Organization name HuGeF
Street address Via Nizza 52
City Torino
State/province Italy
ZIP/Postal code 10126
Country Italy
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (6)
GSM1395157 Snail-ER ESC 0h
GSM1395158 Snail-ER ESC 2h
GSM1395159 Snail-ER ESC 4h
BioProject PRJNA248261

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57854_RAW.tar 15.8 Mb (http)(custom) TAR
GSE57854_non-normalized.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data provided as supplementary file
Raw data are available on Series record

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