|
Status |
Public on Aug 15, 2014 |
Title |
Gene Expression in Effector T cells, wildtype of Thpok-deficient |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
The transcription factor Thpok is essential for CD4 T cell development in the thymus and remains expressed in post-thymic CD4 T cells. We post-thymically inactivated Thpok and compared microarray gene expression in Thpok-deficient CD4 T cells to that in their wildtype CD4 or CD8 counterparts We show that Thpok constrains the transcriptional circuitry to maintain CD4+-lineage integrity in naïve cells and to couple effector differentiation to environmental cues after antigenic stimulation. Redundantly with the related factor LRF, Thpok is continuously needed to prevent the trans-differentiation of mature CD4+ into -CD8+ T cells.
|
|
|
Overall design |
We activated naïve CD4 T cells (either wild-type or Thpok-deficient) and CD8 T cells (wild-type) in vitro under Th1 conditions. Differentiated effectors were sorted 4 days after activation into CD4+CD8- and CD4-CD8+ (wild-type) and CD4+CD8- and CD4+CD8+ (Thpok-deficient) subsets. Total RNA was extracted from sorted subsets and processed for microarray analyses (Affymetrix Mouse Exon 1.0 ST array) at the NCI microarray facility, following the manufacturer’s recommendation. Data is from 3 replicates (except wild-type CD4-CD8+ cells, for which two samples only were processed), generated from two distinct cell preparations.
|
|
|
Contributor(s) |
Wang L, Bosselut R |
Citation(s) |
25129370 |
Submission date |
May 20, 2014 |
Last update date |
Mar 06, 2018 |
Contact name |
David Salomon |
E-mail(s) |
salomond@mail.nih.gov
|
Organization name |
NIH
|
Department |
NCI
|
Street address |
1050 Boyles Drive, Bldg. 560 Room 1246
|
City |
Frederick |
State/province |
MD |
ZIP/Postal code |
21701 |
Country |
USA |
|
|
Platforms (1) |
GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
|
Samples (11)
|
|
Relations |
BioProject |
PRJNA248219 |