|
| Status |
Public on Oct 23, 2014 |
| Title |
Genome-wide expression profiles in young and old mouse liver [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Aging is accompanied by physiological impairments, which, in insulin-responsive tissues, including the liver, predispose individuals to metabolic disease. However, the molecular mechanisms underlying these changes remain largely unknown. Here, we analyze genome-wide profiles of RNA and chromatin organization in the liver of young (3 months) and old (21 months) mice. Transcriptional changes suggest that de-repression of the nuclear receptors PPARα, PPARγ, and LXRα in aged mouse liver leads to activation of targets regulating lipid synthesis and storage, whereas age-dependent changes in nucleosome occupancy are associated with binding sites for both known regulators (forkhead factors and nuclear receptors) and for novel candidates associated with nuclear lamina (Hdac3 and Srf) implicated to govern metabolic function of aging liver. Winged-helix factor Foxa2 and nuclear receptor co-repressor Hdac3 exhibit reciprocal binding pattern at PPARα targets contributing to gene expression changes that lead to steatosis in aged liver.
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| |
|
| Overall design |
Genome-wide expression profiles (RNA-Seq) from young (3 months) and old (21 months) mouse livers
|
| |
|
| Contributor(s) |
Bochkis I, Regev A |
| Citation(s) |
25437555 |
| |
| Submission date |
May 19, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Irina Bochkis |
| E-mail(s) |
imb3q@virginia.edu
|
| Phone |
434-982-6752
|
| Organization name |
University of Virginia
|
| Department |
Pharmacology
|
| Street address |
1340 Jefferson Park Ave
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE58006 |
Changes in nucleosome occupancy associated with metabolic alterations in aged mammalian liver |
|
| Relations |
| BioProject |
PRJNA248136 |
| SRA |
SRP042091 |
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