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Series GSE57762 Query DataSets for GSE57762
Status Public on May 17, 2014
Title Methylation Changes in Soybean Cotyledon Stage Seed Parts
Organism Glycine max
Experiment type Methylation profiling by high throughput sequencing
Summary Seeds are comprised of three major parts of distinct parental origin: the seed coat, embryo, and endosperm. The maternally-derived seed coat is important for nurturing and protecting the seeds during development. By contrast, the embryo and the endosperm are derived from a double fertilization event, where one sperm fertilizes the egg to form the diploid zygote and the other sperm fertilizes the central cell to form the triploid endosperm. Each seed part undergoes distinct developmental programs during seed development. What methylation changes occur in the different seed parts, if any, remains unknown. To uncover the possible role of DNA methylation in different parts of the seed, we characterized the methylome of three major parts of cotyledon stage seeds, the seed coat, embryonic cotyledons, and embryonic axis, using Illumina sequencing.
Overall design Illumina sequencing of bisulfite-converted genomic DNA from three parts of soybean cotyledon stage seeds: seed coat (COT-SC), embryonic cotyledons (COT-COT), and embryonic axis (COT-AX).
Contributor(s) Goldberg RB, Chen M, Lin JY, Hur J, Le BH
Citation(s) 29078418
Submission date May 16, 2014
Last update date Jul 19, 2019
Contact name Bob Goldberg
Phone 310-825-3270
Organization name University of California, Los Angeles
Department Molecular, Cell and Developmental Biology
Street address 610 Charles E Young Drive East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL15008 Illumina HiSeq 2000 (Glycine max)
Samples (3)
GSM1388175 Cotyledon-stage seed coat
GSM1388176 Cotyledon-stage embryonic cotyledons
GSM1388177 Cotyledon-stage embryonic axis
BioProject PRJNA248015
SRA SRP042052

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Supplementary file Size Download File type/resource
GSE57762_RAW.tar 2.9 Gb (http)(custom) TAR (of TXT)
GSE57762_README.txt 673 b (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file

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