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Series GSE57755 Query DataSets for GSE57755
Status Public on May 17, 2014
Title Methylation Changes in Arabidopsis Mature Green Seed Parts
Organism Arabidopsis thaliana
Experiment type Methylation profiling by high throughput sequencing
Summary Seeds are comprised of three major parts of distinct parental origin: the seed coat, embryo, and endosperm. The maternally-derived seed coat is important for nurturing and protecting the seeds during development. By contrast, the embryo and the endosperm are derived from a double fertilization event, where one sperm fertilizes the egg to form the diploid zygote and the other sperm fertilizes the central cell to form the triploid endosperm. Each seed part undergoes distinct developmental programs during seed development. What methylation changes occur in the different seed parts, if any, remains unknown. To uncover the possible role of DNA methylation in different parts of the seed, we characterized the methylome of two major parts of Arabidopsis mature green stage seeds, the seed coat and embryo, using Illumina sequencing.
 
Overall design Illumina sequencing of bisulfite-converted genomic DNA from two parts of Arabidopsis mature green seeds: seed coat (SC) and embryo (EMB).
 
Contributor(s) Goldberg RB, Chen M, Lin JY, Le BH
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Submission date May 16, 2014
Last update date Dec 23, 2018
Contact name Bob Goldberg
E-mail bobglab@mcdb.ucla.edu
Phone 310-825-3270
Organization name University of California, Los Angeles
Department Molecular, Cell and Developmental Biology
Street address 610 Charles E Young Drive East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
Samples (2)
GSM1388111 Mature green stage seed coat
GSM1388112 Mature green stage embryo
Relations
BioProject PRJNA248004
SRA SRP042049

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57755_RAW.tar 301.1 Mb (http)(custom) TAR (of TXT)
GSE57755_README.txt 541 b (ftp)(http) TXT
Raw data are available in SRA
Processed data provided as supplementary file

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