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| Status |
Public on Jul 29, 2014 |
| Title |
Effects of (-)-cannabidiol on human sebocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In the current study, we aimed at exploring the effects of the major non-psychotropic phytocannabinoid of Cannabis sativa, (-)-cannabidiol (CBD), on human sebaceous gland functions. We found that CBD behaved as a highly effective anti-acne agent, targeting all the three key, sebocyte-specific steps of the pathogenesis (i.e. without compromising viability or basal sebaceous lipid production, CBD normalized pro-acne agents-induced excessive lipid synthesis, reduced proliferation and exerted anti-inflammatory actions). The goal of the present microarray analyses was to identify signaling pathways and target genes being involved in mediating the above beneficial effects of CBD.
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| Overall design |
Microarray analyses were performed by using three independent sets of control (vehicle-treated) and CBD-treated (10 μM, 24-hr) human, immortalized SZ95 sebocytes. The first two sets of the samples were analyzed by two-color microarrays (“rep1” and “rep2”). Later on, the RNA from the second sample set was used for the “rep2-1” hybridization (techical repeat). Finally, we analyzed the third (biological) sample set by using one-color microarray (“rep3”).
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| Contributor(s) |
Oláh A, Tóth BI, Borbíró I, Sugawara K, Szöllősi AG, Czifra G, Pál B, Ambrus L, Kloepper J, Camera E, Ludovici M, Picardo M, Voets T, Zouboulis CC, Paus R, Bíró T, Gézsi A |
| Citation(s) |
25061872 |
| Submission date |
May 12, 2014 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Attila Oláh |
| E-mail(s) |
olah.lcmp@gmail.com
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| Organization name |
University of Debrecen
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| Department |
Department of Physiology
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| Street address |
Nagyerdei krt. 98.
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| City |
Debrecen |
| ZIP/Postal code |
H-4032 |
| Country |
Hungary |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (5)
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| GSM1385013 |
Human SZ95 sebocytes_control vs 10 μM CBD-treated (24hrs)_rep1 |
| GSM1385014 |
Human SZ95 sebocytes_control vs 10 μM CBD-treated (24hrs)_rep2 |
| GSM1385015 |
Human SZ95 sebocytes_control vs 10 μM CBD-treated (24hrs)_rep2-1 (technical repeat of rep2) |
| GSM1385016 |
Human SZ95 sebocytes_control vs 10 μM CBD-treated (24hrs)_CONTROL_rep3 |
| GSM1385017 |
Human SZ95 sebocytes_control vs 10 μM CBD-treated (24hrs)_CBD_rep3 |
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| Relations |
| BioProject |
PRJNA246719 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE57571_RAW.tar |
69.8 Mb |
(http)(custom) |
TAR (of TXT) |
| Raw data provided as supplementary file |
| Processed data included within Sample table |
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