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Series GSE57571 Query DataSets for GSE57571
Status Public on Jul 29, 2014
Title Effects of (-)-cannabidiol on human sebocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In the current study, we aimed at exploring the effects of the major non-psychotropic phytocannabinoid of Cannabis sativa, (-)-cannabidiol (CBD), on human sebaceous gland functions. We found that CBD behaved as a highly effective anti-acne agent, targeting all the three key, sebocyte-specific steps of the pathogenesis (i.e. without compromising viability or basal sebaceous lipid production, CBD normalized pro-acne agents-induced excessive lipid synthesis, reduced proliferation and exerted anti-inflammatory actions). The goal of the present microarray analyses was to identify signaling pathways and target genes being involved in mediating the above beneficial effects of CBD.
 
Overall design Microarray analyses were performed by using three independent sets of control (vehicle-treated) and CBD-treated (10 μM, 24-hr) human, immortalized SZ95 sebocytes. The first two sets of the samples were analyzed by two-color microarrays (“rep1” and “rep2”). Later on, the RNA from the second sample set was used for the “rep2-1” hybridization (techical repeat). Finally, we analyzed the third (biological) sample set by using one-color microarray (“rep3”).
 
Contributor(s) Oláh A, Tóth BI, Borbíró I, Sugawara K, Szöllősi AG, Czifra G, Pál B, Ambrus L, Kloepper J, Camera E, Ludovici M, Picardo M, Voets T, Zouboulis CC, Paus R, Bíró T, Gézsi A
Citation(s) 25061872
Submission date May 12, 2014
Last update date Feb 22, 2018
Contact name Attila Oláh
E-mail(s) olah.lcmp@gmail.com
Organization name University of Debrecen
Department Department of Physiology
Street address Nagyerdei krt. 98.
City Debrecen
ZIP/Postal code H-4032
Country Hungary
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (5)
GSM1385013 Human SZ95 sebocytes_control vs 10 μM CBD-treated (24hrs)_rep1
GSM1385014 Human SZ95 sebocytes_control vs 10 μM CBD-treated (24hrs)_rep2
GSM1385015 Human SZ95 sebocytes_control vs 10 μM CBD-treated (24hrs)_rep2-1 (technical repeat of rep2)
Relations
BioProject PRJNA246719

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57571_RAW.tar 69.8 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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