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Series GSE5754 Query DataSets for GSE5754
Status Public on Jan 08, 2007
Title Expression Level Polymorphism Project (ELP) - Est
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary This experiment was donated by The ELP Project website at that was supported in part by the Arabidopsis 2010 project, NSF Division of Molecular and Cellular Biosciences, award 0115109.
The study of natural genetic variation for plant disease resistance responses is a complementary approach to utilizing mutants to elucidate genetic pathways. While some key genes involved in pathways controlling disease resistance, and signaling intermediates such as salicylic acid and jasmonic acid, have been identified through mutational analyses, the use of genetic variation in natural populations permits the identification of change-of-function alleles, which likely act in a quantitative manner. Whole genome microarrays, such as Affymetrix GeneChips, allow for molecular characterization of the disease response at a genomics level and characterization of differences in gene expression due to natural variation. Differences in the level of gene expression, or expression level polymorphisms (ELPs), can be mapped in a segregating population to identify regulatory quantitative trait loci (expression QTLs) affecting host resistance responses. In order to identify an appropriate RIL population to map QTL controlling disease resistance responses, we performed a parental survey of 7 different Arabidopsis accessions.
We treated vegetatively grown plants with either salicylic acid or a control solution, and harvested the plants at 3 different time points after chemical treatment. We present Affymetrix GeneChip microarray expression data for 3 biological replications of this parental survey.
Keywords: strain_or_line; compound_treatment; time_series
Overall design 18 samples were used in this experiment
Contributor(s) St. Clair D, Townsend H, Emmerson Z, Schildknecht B
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Submission date Sep 01, 2006
Last update date Aug 28, 2018
Contact name Nottingham Arabidopsis Stock Centre (NASC)
Phone +44 (0)115 951 3237
Fax +44 (0)115 951 3297
Organization name Nottingham Arabidopsis Stock Centre (NASC)
Department School of Biosciences, University of Nottingham
Street address Sutton Bonington Campus
City Loughborough
ZIP/Postal code LE12 5RD
Country United Kingdom
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (18)
GSM134376 St.Clair_1-37_290_Est_0.02%-silwet_Rep1_ATH1
GSM134377 St.Clair_1-38_332_Est_0.02%-silwet_Rep2_ATH1
GSM134378 St.Clair_1-39_394_Est_0.02%-silwet_Rep3_ATH1
BioProject PRJNA97081

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5754_RAW.tar 39.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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