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Series GSE5751 Query DataSets for GSE5751
Status Public on Jan 08, 2007
Title The early post-germinative embryo and endosperm transcriptomes in Arabidopsis
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Arabidopsis seed germination is coordinated with the strong induction of metabolic pathways required for the mobilisation and utilization of seed storage reserves. These are essential to support the seedling before the establishment of photoauxotrophic growth. The activity of genes encoding enzymes required for lipid mobilisation is regulated largely at the level of transcription, but our knowledge of how this regulation occurs is extremely limited. After germination the rate of lipid reserve mobilisation is determined by the carbohydrate status of the seedling and by the osmotic potential of the growth substrate. The plant response to both of these requires the action of the hormone abscisic acid (ABA).
We have shown that this regulation is tissue specific (Penfield et al., 2004 Plant Cell 16, 2705-2718), and that although lipid breakdown in the embryo is inhibited by ABA, lipid breakdown in the endosperm tissues is not. Furthermore, in many species the action of the endosperm is central to the processes controlling seed germination, yet very little is known about gene expression in this tissue, or of the function of the endosperm in mature Arabidopsis seeds. Mature Arabidopsis seeds can be dissected into embryo and endosperm/seed coat fractions, with the latter fraction containing RNA only from the endosperm as the seed coat cells undergo programmed cell death during the latter stages of seed development. In this experiment we divide seeds into embryo and endosperm tissues and transcript profile both shortly after germination, or when germination and lipid reserve mobilisation are inhibited by ABA or by the gibberellin biosynthesis inhibitor paclobutrazol. In this way we can discover the endosperm transcriptome and uncover candidate regulators of lipid mobilisation by searching for genes showing differential expression between embryo and endosperm before and after ABA treatment.

Experimenter name = Steven Penfield
Experimenter phone = 01904 328759
Experimenter fax = 01904 328762
Experimenter department = Department of Biology
Experimenter institute = Centre for Novel Agricultural Products
Experimenter address = University of York
Experimenter address = PO BOX 373
Experimenter address = York
Experimenter zip/postal_code = YO10 5YW
Experimenter country = UK
Keywords: organism_part_comparison_design
Overall design 18 samples were used in this experiment
Contributor(s) Penfield S, Graham I, Townsend H, Emmerson Z, Schildknecht B
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Submission date Sep 01, 2006
Last update date Aug 28, 2018
Contact name Nottingham Arabidopsis Stock Centre (NASC)
Phone +44 (0)115 951 3237
Fax +44 (0)115 951 3297
Organization name Nottingham Arabidopsis Stock Centre (NASC)
Department School of Biosciences, University of Nottingham
Street address Sutton Bonington Campus
City Loughborough
ZIP/Postal code LE12 5RD
Country United Kingdom
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (18)
GSM134299 Penfield_1-1_endosperm-control_Rep1_ATH1
GSM134300 Penfield_1-2_endosperm-control_Rep2_ATH1
GSM134301 Penfield_1-3_endosperm-control_Rep3_ATH1
Affiliated with GSE69995
BioProject PRJNA97075

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5751_RAW.tar 36.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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