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Series GSE5750 Query DataSets for GSE5750
Status Public on Jan 08, 2007
Title Growth of suspension-cultured cells
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary The growth of Arabidopsis cell cultures following their sub-culture into fresh media follows standard growth kinetics of a period of exponential increase associated with high rate of cell division, followed by a slowing of the rate of increase as cells approach stationary phase. For the analysis described here, MM2d cells were subcultured into fresh MSS-medium and samples were taken at day 1, day3, day 5 and day7. We have carried out transcriptional profiling analysis with the aim to follow growth stage specific gene expression during unperturbed growth using the near full genome ATH1 arrays (Menges et al., 2003).
Journal Absract:
(Plant Molecular Biology: 53, 2003)
Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platform by means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during synchronous cell cycle re-entry and during synchronous cell cycle progression provides a unique integrated view of gene expression responses in a higher-plant system. Particularly striking is that expression of over 14 000 genes belonging to all defined categories can be reliably detected, suggesting that integrated and comparative analysis of data sets derived from transcript profiling of cultures is a powerful approach to identify candidate components involved in a wide range of biological processes. Combinatorial analysis of independent cell cycle synchrony methods allows the identification of genes that are apparently cell-cycle-regulated but are most likely responding to the induction of synchrony. We thus present an integrated genome-wide view of the transcriptional profile of a plant suspension culture and identify a refined set of 1082 cell cycle regulated genes largely independent of synchrony method.

Experimenter name = Jim Murray
Experimenter phone = 44 1223 334166
Experimenter fax = 44 1223 334162
Experimenter department = J Murray Laboratory
Experimenter address = University of Cambridge
Experimenter address = Institute of Biotechnology
Experimenter address = Tennis Court Road
Experimenter address = Cambridge
Experimenter zip/postal_code = CB2 1QT
Experimenter country = United Kingdom
Keywords: time_series_design
Overall design 4 samples were used in this experiment
Contributor(s) Murray J, Townsend H, Emmerson Z, Schildknecht B
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Submission date Sep 01, 2006
Last update date Aug 28, 2018
Contact name Nottingham Arabidopsis Stock Centre (NASC)
Phone +44 (0)115 951 3237
Fax +44 (0)115 951 3297
Organization name Nottingham Arabidopsis Stock Centre (NASC)
Department School of Biosciences, University of Nottingham
Street address Sutton Bonington Campus
City Loughborough
ZIP/Postal code LE12 5RD
Country United Kingdom
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (4)
GSM134202 Murray_3-1_D1-GROWTH_Rep1_ATH1
GSM134204 Murray_3-2_D3-GROWTH_Rep1_ATH1
GSM134206 Murray_3-3_D5-GROWTH_Rep1_ATH1
BioProject PRJNA97073

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5750_RAW.tar 9.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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