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Series GSE5748 Query DataSets for GSE5748
Status Public on Jan 08, 2007
Title In vitro tracheary element transdifferentiation of Col-0 suspension cells.
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary This experiment was annotated by TAIR (
Col-0 suspension cells provided by Dr. C. Koncz and Dr. M. Umeda. 15 ml of Arabidopsis Col-0 suspension cells (Mathur et al. 1998) was transferred to 35 ml of a fresh modified Murashige and Skoog (MS) medium supplemented with 1 ug /ml 2,4-dichlorphenoxyacetic acid and 3% sucrose every 7 day, and cultured on a rotary shaker at 120 rpm in the dark at 22 C for subculture. For xylem vessel element induction, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3, and cultured as described above. The frequency of xylem vessel element formation was calculated as the proportion of xylem vessel elements to the number of living cells and the vessel elements.

Experimenter name = Hiroo Fukuda
Experimenter phone = +81-3-5841-4463
Experimenter fax = +81-3-5841-4462
Experimenter department = H Fukuda Laboratory
Experimenter institute = University of Tokyo
Experimenter address = Department of Biological Science
Experimenter address = 2nd Buil.
Experimenter address = Hongo 7-3-1, Bunkyo-ku
Experimenter zip/postal_code = Tokyo 113-0033
Experimenter country = Tokyo
Keywords: compound_treatment_design; time_series_design;
Overall design 12 samples were used in this experiment
Contributor(s) Fukuda H, Townsend H, Emmerson Z, Schildknecht B
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Submission date Sep 01, 2006
Last update date Aug 28, 2018
Contact name Nottingham Arabidopsis Stock Centre (NASC)
Phone +44 (0)115 951 3237
Fax +44 (0)115 951 3297
Organization name Nottingham Arabidopsis Stock Centre (NASC)
Department School of Biosciences, University of Nottingham
Street address Sutton Bonington Campus
City Loughborough
ZIP/Postal code LE12 5RD
Country United Kingdom
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (12)
GSM133956 Fukuda_1-1_0A_Rep1_ATH1
GSM133957 Fukuda_1-2_0B_Rep2_ATH1
GSM133958 Fukuda_1-3_2A_Rep1_ATH1
BioProject PRJNA97069

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5748_RAW.tar 27.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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