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Series GSE57398 Query DataSets for GSE57398
Status Public on Feb 20, 2015
Title Genome-wide characterization of HSF1 binding in human WT and FBXW7 KO colon cancer cells under basal conditions and upon heat shock
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary FBXW7 modulates stress response by post-translational modification of HSF1
HSF1 orchestrates the heat-shock response upon exposure to heat stress and activates a transcriptional program vital for cancer cells. In this study we assayed for genome-wide localization of HSF1 enrichment in the HCT116 FBXW7 KO colon cells and their wild type counterpart in untreated cells and upon heat shock. These results revealed that accumulation of nuclear HSF1 in FBXW7 KO cells results in rewiring of the HSF1 transcriptional program.
 
Overall design Five million cells were used for the ChIP and precipitated using 5 micrograms of antibody (cell signaling, 4356) against human HSF1
 
Contributor(s) Kourtis N
Citation(s) 25720964
Submission date May 07, 2014
Last update date May 15, 2019
Contact name Nikos Kourtis
E-mail(s) nkourtis@gmail.com
Organization name NYU School of Medicine
Street address 550 First Avenue
City New York
ZIP/Postal code 10016
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (10)
GSM1382049 ChIP-Seq analysis of HSF1 in HCT116 FBXW7 KO cells upon heat shock treatment replicate1
GSM1382050 ChIP-Seq analysis of HSF1 in HCT116 FBXW7 KO cells upon heat shock treatment replicate2
GSM1382051 Sequencing analysis of input in HCT116 FBXW7 KO cells upon heat shock treatment
This SubSeries is part of SuperSeries:
GSE57399 FBXW7 modulates stress response by post-translational modification of HSF1
Relations
BioProject PRJNA246392
SRA SRP041754

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57398_RAW.tar 130.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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