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| Status |
Public on Feb 20, 2015 |
| Title |
Genome-wide characterization of HSF1 binding in human WT and FBXW7 KO colon cancer cells under basal conditions and upon heat shock |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
FBXW7 modulates stress response by post-translational modification of HSF1
HSF1 orchestrates the heat-shock response upon exposure to heat stress and activates a transcriptional program vital for cancer cells. In this study we assayed for genome-wide localization of HSF1 enrichment in the HCT116 FBXW7 KO colon cells and their wild type counterpart in untreated cells and upon heat shock. These results revealed that accumulation of nuclear HSF1 in FBXW7 KO cells results in rewiring of the HSF1 transcriptional program.
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| Overall design |
Five million cells were used for the ChIP and precipitated using 5 micrograms of antibody (cell signaling, 4356) against human HSF1
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| Contributor(s) |
Kourtis N |
| Citation(s) |
25720964 |
| |
| Submission date |
May 07, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Nikos Kourtis |
| E-mail(s) |
nkourtis@gmail.com
|
| Organization name |
NYU School of Medicine
|
| Street address |
550 First Avenue
|
| City |
New York |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (10)
|
| GSM1382049 |
ChIP-Seq analysis of HSF1 in HCT116 FBXW7 KO cells upon heat shock treatment replicate1 |
| GSM1382050 |
ChIP-Seq analysis of HSF1 in HCT116 FBXW7 KO cells upon heat shock treatment replicate2 |
| GSM1382051 |
Sequencing analysis of input in HCT116 FBXW7 KO cells upon heat shock treatment |
| GSM1382052 |
ChIP-Seq analysis of HSF1 in HCT116 FBXW7 KO untreated cells replicate1 |
| GSM1382053 |
Sequencing analysis of input in HCT116 FBXW7 KO untreated cells |
| GSM1382054 |
ChIP-Seq analysis of HSF1 in HCT116 WT cells upon heat shock treatment replicate1 |
| GSM1382055 |
ChIP-Seq analysis of HSF1 in HCT116 WT cells upon heat shock treatment replicate2 |
| GSM1382056 |
Sequencing analysis of input in HCT116 WT cells upon heat shock treatment |
| GSM1382057 |
ChIP-Seq analysis of HSF1 in HCT116 WT untreated cells replicate1 |
| GSM1382058 |
Sequencing analysis of input in HCT116 WT untreated cells |
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| This SubSeries is part of SuperSeries: |
| GSE57399 |
FBXW7 modulates stress response by post-translational modification of HSF1 |
|
| Relations |
| BioProject |
PRJNA246392 |
| SRA |
SRP041754 |
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