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Series GSE5736 Query DataSets for GSE5736
Status Public on Jan 08, 2007
Title To identify changes in gene expression during silique senescence in Arabidopsis thaliana
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary The aim of the experiment is to compare and contrast the profiles of gene expression during silique senescence and to identify the molecular events that regulate the timing of the process. Silique occurs over a highly predictable time frame and as a consequence it may be easier to identify the molecular events that are critical for the process to take place than in systems such as leaf senescence. The material will be collected from 20 Arabidopsis thaliana ecotype Columbia plants (NASC order number N1093) grown under controlled conditions specified under NASC protocol: Baseline sample preparation. Two pods will be collected from each plant and pooled from 20 plants to reduce sampling variability. It is intended that 3 replicates of the experiment will be submitted. The RNA will be extracted from mature green silique tissues of 10 days after anthesis and senescing pod tissues of 20 days after anthesis using lithium chloride extraction protocol and cleaned with Qiagen RNAeasy column.

Experimenter name = Thomas Yang
Experimenter phone = 01159516378
Experimenter institute = University of Nottingham
Experimenter address = Division of Plant Sciences
Experimenter address = University of Nottingham
Experimenter address = Sutton Bonington Campus
Experimenter address = Loughborough
Experimenter address = Leicestershire
Experimenter zip/postal_code = LE12 5RD
Experimenter country = UK
Keywords: development_or_differentiation_design
Overall design 6 samples were used in this experiment
Contributor(s) Yang T, Roberts J, Townsend H, Emmerson Z, Schildknecht B
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Submission date Sep 01, 2006
Last update date Aug 28, 2018
Contact name Nottingham Arabidopsis Stock Centre (NASC)
Phone +44 (0)115 951 3237
Fax +44 (0)115 951 3297
Organization name Nottingham Arabidopsis Stock Centre (NASC)
Department School of Biosciences, University of Nottingham
Street address Sutton Bonington Campus
City Loughborough
ZIP/Postal code LE12 5RD
Country United Kingdom
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (6)
GSM133816 Yang_1-1_young-pod_Rep1_ATH1
GSM133817 Yang_1-2_old-pod_Rep1_ATH1
GSM133818 Yang_1-3_young-pod_Rep2_ATH1
BioProject PRJNA97045

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5736_RAW.tar 13.0 Mb (http)(custom) TAR (of CEL)

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