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Series GSE5730 Query DataSets for GSE5730
Status Public on Jan 08, 2007
Title Transcriptional profiling of laser-capture micro-dissected embryonic tissues
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary The aim of this BBSRC-funded project is to develop laser-capture microdissection (LCMD) to isolate small cell clusters in different regions of arabidopsis embryos at different stages of development; to develop RNA amplification procedures on dissected tissue sampes; and to use DNA microarray techniques to investigate global transcriptional differences between samples. Cryosectioned embryos of ecotype Col-O of globular, heart and torpedo stage were used to isolate cell clusters from the apical and basal regions, for RNA isolation and amplification. !Samples will be provided as T7-primed cDNA, with three biological replicates for each tissue to be analysed. Each replicate comprises cDNA from pooled tissue samples from ca. 15 embryos. The experimental details have been discussed with Sean May et al. at NASC.

Experimenter name = Stuart Casson and Matthew Spencer
Experimenter phone = 0191 374 7356
Experimenter fax = 0191 374 2417
Experimenter institute = Durham University
Experimenter address = Integrative Cell Biology Laboratory
Experimenter address = School of Biological Sciences
Experimenter address = Durham University
Experimenter address = South Road
Experimenter address = Durham
Experimenter zip/postal_code = DH1 3LE
Experimenter country = UK
Keywords: organism_part_comparison_design; development_or_differentiation_design
Overall design 27 samples were used in this experiment
Contributor(s) Casson S, Spencer M, Lindsey K, Townsend H, Emmerson Z, Schildknecht B
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Submission date Sep 01, 2006
Last update date Aug 28, 2018
Contact name Nottingham Arabidopsis Stock Centre (NASC)
Phone +44 (0)115 951 3237
Fax +44 (0)115 951 3297
Organization name Nottingham Arabidopsis Stock Centre (NASC)
Department School of Biosciences, University of Nottingham
Street address Sutton Bonington Campus
City Loughborough
ZIP/Postal code LE12 5RD
Country United Kingdom
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (27)
GSM133755 Lindsey_1-7_heart-stage-cotyledon_Rep1_ATH1
GSM133756 Lindsey_1-10_heart-stage-root_Rep1_ATH1
GSM133757 Lindsey_1-8_heart-stage-cotyledon_Rep2_ATH1
Affiliated with GSE69995
BioProject PRJNA97035

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5730_RAW.tar 54.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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