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Series GSE56796 Query DataSets for GSE56796
Status Public on Jul 01, 2014
Title CORTECON: A Temporal Transcriptome Analysis of In Vitro Human Cerebral Cortex Development From Human Embryonic Stem Cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Many neurological and psychiatric disorders affect the cerebral cortex, and a clearer understanding of the molecular processes underlying human corticogenesis will provide greater insight into such pathologies. To date, knowledge of gene expression changes accompanying corticogenesis is largely based on murine data. Here we present a searchable, comprehensive, temporal gene expression dataset encompassing cerebral cortical development from human embryonic stem cells (hESCs). Using a modified differentiation protocol and RNA-Seq technology with computational analysis, we identified sets of genes and long non-coding RNAs that significantly change during corticogenesis, and those enriched for disease-associations. Numerous alternatively-spliced genes with varying temporal patterns of expression are revealed, including TGIF1, involved in holoprosencephaly and MARK1, involved in autism. We have created a database (http://cortecon.neuralsci.org) that provides online, query-based access to changes in RNA expression and alternatively spliced transcripts during human cortical development.
 
Overall design Nine timepoints (days 0,7,12,19,26,33,49,63,77) covering human corticogenesis from embyronic stem cells.
 
Contributor(s) Boles NC, van de Leemput J, Temple S, Fasano CA
Citation(s) 24991954
Submission date Apr 15, 2014
Last update date Mar 20, 2019
Contact name Thomas R Kiehl
E-mail tomkiehl@neuralsci.org
Phone 518-694-8188
Organization name Neural Stem Cell Institute
Street address One Discovery Drive
City Rensselaer
State/province NY
ZIP/Postal code 12144
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (44)
GSM1369182 Day 0 A
GSM1369183 Day 0 B
GSM1369184 Day 0 C
Relations
BioProject PRJNA244622
SRA SRP041179

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE56796_Cortecon_normalized.csv.gz 3.7 Mb (ftp)(http) CSV
GSE56796_Test_normalized.csv.gz 2.9 Mb (ftp)(http) CSV
Raw data are available in SRA
Processed data is available on Series record

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