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Series GSE5679 Query DataSets for GSE5679
Status Public on Sep 19, 2006
Title Comparative gene expression profile of PPARg and RARa ligand treated human dendritic cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Our data indicated that activation of the PPARg nuclear receptor induces a retinoid response in human dendritic cells. In order to assess the contribution of retinoid signaling to the PPARg response we decided to use a combination of pharmacological activators and inhibitors of these pathways. Cells were treated with the synthetic PPARg ligand rosiglitazone (RSG), or with RSG along with the RARa antagonist (AGN193109) to block RARa mediated gene expression, or the RARa specific agonists (AM580) alone. This design allows one to determine if retinoid signaling is a downstream event of PPARg activation and what portion of PPARg regulated genes are regulated via induced retinoid signaling.
Keywords: ligand response
Overall design Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF, cytokine treatment was repeated at day 3. Ligands were added at the beginning of differentiation but AGN193109 (AGN) treatment was repeated at day 3. Cells were obtained from three healthy individuals (three biological replicates) and cells were treated with vehicle (DC), 2.5 uM rosiglitazone (DC RSG), 100 nM AM580 (DC AM). RSG treatment was also combined with 1 uM AGN193109 (DC RSG AGN) in this case two biological replicates was used (RNA was obtained from 2. and 3. individuals).
Contributor(s) Nagy L, Szatmari I
Citation(s) 16982809
Submission date Aug 31, 2006
Last update date Mar 25, 2019
Contact name Istvan Szatmari
Phone 36 52 416-432
Fax 36 52 314-989
Organization name University of Debrecen
Department Department of Biochemistry and Molecular Biology
Lab Nuclear Hormone Receptor
Street address Egyetem tér 1.
City Debrecen
ZIP/Postal code H-4010
Country Hungary
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (11)
GSM132919 DC1
GSM132921 DC1 RSG
GSM132922 DC1 AM
BioProject PRJNA96965

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Supplementary file Size Download File type/resource
GSE5679_RAW.tar 89.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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