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Series GSE56659 Query DataSets for GSE56659
Status Public on Apr 10, 2014
Title The Arabidopsis spaceflight transcriptome: a comparison of whole plants to discrete root, hypocotyl and shoot responses to the orbital environment
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary transcriptome response of Arabidopsis cultivar Columbia and WS whole plants and plant tissue roots, hypocotyls and shoots to the spaceflight environment
Arabidopsis thaliana was evaluated for its response to the spaceflight environment in three replicated experiments on the International Space Station. Two approaches were used; GFP reporter genes were used to collect gene expression data in real time within unique GFP imaging hardware, and plants were harvested on orbit to RNAlater for subsequent analyses of gene expression with using Affymetrix and SAGE transcriptome analyses. Three tissue types were examined (leaves, hypocotyls and roots) and compared to analyses conducted with whole plants. Transcriptome analyses with whole plants suggested that the spaceflight environment had little impact on the transcriptome of arabidopsis, however, closer examination of selected tissues revealed that there are a number of tissue-specific responses that arabidopsis employs to respond to this novel environment.

This experiment was launched on STS-131 in 2010 and was supported by NASA grant NNX07AH27G - Transgenic Plant Biomonitors of Space Flight Exposure to R.J. Ferl and A-L. Paul.
 
Overall design whole plants total samples 6: spaceflight 3 samples vs unit gravity ground control' 3 samples; plant tissue total samples 30: shoots: spaceflight 5 samples vs unit gravity ground control' 5 samples; hypocotyls: spaceflight 5 samples vs unit gravity ground control' 5 samples; roots: spaceflight 5 samples vs unit gravity ground control' 5 samples;
 
Contributor(s) Paul A, Ferl RJ, Zupanska AK, Zhang Y, Sun Y
Citation(s) 23919896
Submission date Apr 09, 2014
Last update date Jun 12, 2017
Contact name Robert J. Ferl
E-mail ferllabuf@gmail.com
Phone 352-273-8030
Organization name University of Florida
Department Horticultural Sciences
Lab Ferl's lab
Street address 1301 Fifield Hall PO Box 110690
City Gainesville
State/province Florida
ZIP/Postal code 32611
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (36)
GSM1366455 FLIGHT TAGES 2B plate2
GSM1366456 FLIGHT TAGES 2B plate4
GSM1366457 FLIGHT TAGES 2B plate6
Relations
BioProject PRJNA244318

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE56659_RAW.tar 59.7 Mb (http)(custom) TAR (of CEL)
GSE56659_result_MAS5_Hypocotyls.txt.gz 4.9 Kb (ftp)(http) TXT
GSE56659_result_MAS5_Leaves.txt.gz 7.0 Kb (ftp)(http) TXT
GSE56659_result_MAS5_Root.txt.gz 7.7 Kb (ftp)(http) TXT
GSE56659_result_MAS5_files_content_description.txt.gz 638 b (ftp)(http) TXT
GSE56659_result_MAS5_whole_plants.txt.gz 1.4 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data is available on Series record

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