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Status |
Public on Apr 10, 2014 |
Title |
Transcription profiling by array of the response of Arabidopsis cultivar Columbia etiolated seedlings and undifferentiated tissue culture cells to the spaceflight environment |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
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Summary |
transcriptome response of Arabidopsis cultivar Columbia etiolated seedlings and undifferentiated tissue culture cells to the spaceflight environment We address a key baseline question of whether gene expression changes are induced by the orbital environment, and then we ask whether undifferentiated cells, cells presumably lacking the typical gravity response mechanisms, perceive spaceflight. Arabidopsis seedlings and undifferentiated cultured Arabidopsis cells were launched in April, 2010, as part of the BRIC-16 flight experiment on STS-131. Biologically replicated DNA microarray and averaged RNA digital transcript profiling revealed several hundred genes in seedlings and cell cultures that were significantly affected by launch and spaceflight. The response was moderate in seedlings; only a few genes were induced by more than 7-fold, and the overall intrinsic expression level for most differentially expressed genes was low. In contrast, cell cultures displayed a more dramatic response, with dozens of genes showing this level of differential expression, a list comprised primarily of heat shock-related and stress-related genes. This baseline transcriptome profiling of seedlings and cultured cells confirms the fundamental hypothesis that survival of the spaceflight environment requires adaptive changes that are both governed and displayed by alterations in gene expression. The comparison of intact plants with cultures of undifferentiated cells confirms a second hypothesis: undifferentiated cells can detect spaceflight in the absence of specialized tissue or organized developmental structures known to detect gravity.
This experiment was launched on STS-131 in 2010 and was supported by NASA grant NNX10AF45G - The Impact of Spaceflight on Arabidopsis: Deep Sequencing and DNA Arrays as Collaborative Readouts of the Transcriptome of Arabidopsis Seedlings and Undifferentiated Cells in Space to A-L. Paul and R.J. Ferl.
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Overall design |
undifferentiated cultured cells total samples 12: spaceflight 6 samples vs unit gravity ground control' 6 samples; etiolated seedlings total samples 12: spaceflight 6 samples vs unit gravity ground control' 6 samples
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Contributor(s) |
Paul A, Ferl RJ, Zupanska AK, Ostrow DT, Zhang Y, Sun Y, Li J |
Citation(s) |
22221117, 23258370 |
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Submission date |
Apr 09, 2014 |
Last update date |
Jun 12, 2017 |
Contact name |
Robert J. Ferl |
E-mail(s) |
ferllabuf@gmail.com
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Phone |
352-273-8030
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Organization name |
University of Florida
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Department |
Horticultural Sciences
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Lab |
Ferl's lab
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Street address |
1301 Fifield Hall PO Box 110690
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City |
Gainesville |
State/province |
Florida |
ZIP/Postal code |
32611 |
Country |
USA |
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Platforms (1) |
GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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Samples (24)
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Relations |
BioProject |
PRJNA244316 |