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Series GSE56508 Query DataSets for GSE56508
Status Public on Dec 30, 2017
Title Expression analysis of Aedes aegypti starins Moyo-S and Moyo-R in response to different serotypes of dengue virus
Organism Aedes aegypti
Experiment type Expression profiling by array
Summary Investigation of whole genome gene expression level changes in Moyo-S and Moyo-R strains of Aedes aegypti after oral infection of serotype 1, serotype 2, serotype 3 and serotype 4 of dengue virus
The Moyo-S is highly suscpetible to dengue infection whereas Moyo-R is refractory to the dengue infection. They have been investigated in our previous studies incluidng Behura et al. (2011). PLoS neglected tropical diseases 5 (11), e1385; and Chauhan et al. (2012). PloS one 7 (10), e47350.
 
Overall design This is a 12-plex high defination NimbleGen array design. Two strains of Aedes aegypti (Moyo-S and Moyo-R) were investigated afteral orally feeding the females with blood meal contamianted with different serotypes (n =4) of dengue virus. Expression levels of Aedes aegypti genes were measured at two post-infection time points 3hour and 18hour. Uninfected control (blood fed only, no dengue) were prepared for each time point for both Moyo-S and Moyo-R. Each control and test sample was derived from three independent feeding experiments. Hybiridization was done in two batches. In the first batch, 12 sample (4 control and 8 test samples) representing infection material with two serotypes were used. In the second batch, the same procedure was used but for the other two serotypes of dengue virus. Hence, a total of 24 samples were used for hybridization.
 
Contributor(s) Behura SK, Severson DW
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Submission date Apr 04, 2014
Last update date Dec 31, 2017
Contact name Susanta K. Behura
E-mail(s) behuras@missouri.edu
Organization name Univeristy of Missouri
Department Division of Animal Sciences
Street address 920 East Campus Drive
City Columbia
State/province Missouri
ZIP/Postal code 65211
Country USA
 
Platforms (1)
GPL18530 NimbleGen Aedes aegypti 12plex array [090305_Aedes_aegypti_TEfam_expr]
Samples (24)
GSM1362955 Moyo-S_Uninfected_control_3hr_post_feeding_batch 1
GSM1362956 Moyo-S_Dengue_Serotype1_infected_3hr_post_feeding_batch 1
GSM1362957 Moyo-S_Dengue_Serotype2_infected_3hr_post_feeding_batch 1
Relations
BioProject PRJNA243563

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE56508_RAW.tar 62.4 Mb (http)(custom) TAR (of PAIR)
Processed data included within Sample table
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