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Series GSE56268 Query DataSets for GSE56268
Status Public on Jun 01, 2014
Title miR-28 expression in the Burkitt lymphoma cell line P3HR1
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Burkitt lymphoma (BL) is a highly aggressive B cell non-Hodgkin lymphoma (B-NHL), which originates from germinal center (GC) B cells and harbors translocations deregulating the MYC oncogene. A comparative analysis of microRNAs (miRNAs) expressed in normal and malignant GC B cells identified miR-28 as significantly down-regulated in BL, as well as in other GC-derived B-NHL. We show that re-expression of miR-28 impairs cell growth and clonogenic properties of BL cells by modulating several targets including MAD2L1, a component of the spindle checkpoint whose down-regulation is essential in mediating miR-28-induced growth-arrest, and BAG1, an activator of the ERK pathway.
 
Overall design P3HR1 Burkitt lymphoma cell line was engineered to display inducible expression of GFP alone or GFP in combination with the miR-28 precursor from the pRTS1 vector upon doxycycline treatment. Two bulk populations (A and B) were established for both the control (GFP alone) and the miR28-expressing (GFP and miR-28 precursor) cells. Cells were induced with 0.1ug/ml doxycycline for 12h or 24h. Induction was performed on each bulk population in triplicates.
 
Contributor(s) Schneider C, Basso K, Holmes AB, Dalla-Favera R
Citation(s) 24843176
Submission date Mar 27, 2014
Last update date Mar 25, 2019
Contact name Riccardo Dalla-Favera
E-mail(s) rd10@cumc.columbia.edu
Organization name Columbia University
Street address 1130 St Nicholas Avenue
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (24)
GSM1358007 P3HR1_CTR_A1_12h
GSM1358008 P3HR1_CTR_A2_12h
GSM1358009 P3HR1_CTR_A3_12h
Relations
BioProject PRJNA242802

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE56268_RAW.tar 118.4 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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