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Series GSE55813 Query DataSets for GSE55813
Status Public on Mar 12, 2014
Title Expression data from amiEBBB1 poplar apices
Platform organism Populus sp.
Sample organism Populus tremula x Populus alba
Experiment type Expression profiling by array
Summary We study gene expression Populus amiEBB1 lines affecting dormancy. We used microarrays to detail the global program of gene expression underlying morphological and developmental changes droved by expression of artifical micro RNA (ami) targeting EBB1 gene.
By screening activation tagging population we identified the EARLY BUD-BREAK1 (EBB1) mutant. We positioned the tag, localized a putative candidate gene and verified transcription activation. The activated gene encodes an AP2/ERF transcription factor similar to a small B1 gene-subfamily in Arabidopsis encoding strong regulators of meristem function and lateral organ outgrowth. We fully recapitulated the phenotype by overexpression of the gene into the same genotype under strong constitutive promoter (P35S::EBB1). We also found that EBB1 transcript abundance in wild type plants increases during the period prior to bud-break, and in response to the hormone cytokinin. Down-regualtion of EBB1 influence negatively bud break. Our data indicates that EBB1 plays a important role in the process of bud-break.
 
Overall design Poplar apex was selected for RNA extraction and hybridization on Affymetrix microarrays. We used apices tissue from amiEBB1 grown greenhouse healthy plants.
 
Contributor(s) Busov VB, Yordanov YS
Citation(s) 24951507
Submission date Mar 11, 2014
Last update date Jan 02, 2015
Contact name Yordan S Yordanov
E-mail(s) ysyordan@mtu.edu
Organization name Michigan Technological University
Department Forest Resources and Environmental Science
Street address 1400 Townsend Drive
City Houghton
State/province MICHIGAN
ZIP/Postal code 49931
Country USA
 
Platforms (1)
GPL4359 [Poplar] Affymetrix Poplar Genome Array
Samples (2)
GSM1346310 amiEBB1_Apex_rep1
GSM1346311 amiEBB1_Apex_rep2
Relations
BioProject PRJNA241043

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55813_RAW.tar 9.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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