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Series GSE55357 Query DataSets for GSE55357
Status Public on Jun 16, 2015
Title Genowide-wide analysis of scc2-4 binding.
Organism Saccharomyces cerevisiae BY4741
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The evolutionarily conserved cohesin complex is crucial for holding sister chromatids together from the time of DNA replication until their segregation during the metaphase to anaphase transition. Human diseases associated with with mutations in the cohesin network are termed "cohesinopathies". Scc2 is required for loading cohesin onto DNA prior to DNA replication. Cornelia de Lange syndrome (CdLS), a developmental disorder characterized by growth and intellectual impairment is caused by mutations in Scc2. How mutations in Scc2 gives rise to these developmental defects is currently unknown, as overt defects in chromosome segregation are not observed in CdLS patients. One hypothesis is that, reduced binding of Scc2 causes gene misregulation. To further explore this idea ChIP-seq was performed using an scc2-4 mutant. We observed from the analysis, we observe reduce binding of Scc2 to Pol II transcribed genes (snoRNAs, ribosomal protein genes) and pol II transcribed genes (tRNAs). Studies are currently underway to examine the biological implications of this observation.
Overall design Examining genome-wide binding of scc2-4 mutant
Contributor(s) Zakari M, Egidy R, Seidel CW, Gerton JL
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Submission date Feb 26, 2014
Last update date May 15, 2019
Contact name Musinu Zakari
Phone 8169264380
Organization name Stowers Institute for Medical Research
Lab Gerton Lab
Street address 1000E 50th Street
City Kansas City
State/province Missouri
ZIP/Postal code 64110
Country USA
Platforms (1)
GPL18334 Illumina HiSeq 2500 (Saccharomyces cerevisiae BY4741)
Samples (8)
GSM1334672 wt_wce_2
GSM1334673 wt_ip_1
GSM1334674 scc2-4_ip_1
This SubSeries is part of SuperSeries:
GSE55358 Genomic analysis of the scc2-4 mutant in budding yeast
BioProject PRJNA239407
SRA SRP038961

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Supplementary file Size Download File type/resource
GSE55357_RAW.tar 242.3 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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