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Series GSE55316 Query DataSets for GSE55316
Status Public on Jun 16, 2015
Title The cohesin loader Scc2 regulates key regulatory genes critical for development
Organism Saccharomyces cerevisiae BY4741
Experiment type Expression profiling by high throughput sequencing
Summary The evolutionarily conserved cohesin complex is crucial for holding sister chromatids together from the time of DNA replication until their segregation during the metaphase to anaphase transition. Human diseases associated with with mutations in the cohesin network are termed "cohesinopathies". Scc2 is required for loading cohesin onto DNA prior to DNA replication. Cornelia de Lange syndrome (CdLS), a developmental disorder characterized by growth and intellectual impairment is caused by mutations in Scc2. How mutations in Scc2 gives rise to these developmental defects is currently unknown, as overt defects in chromosome segregation are not observed in CdLS patients.This has led to the hypothesis that developmental disorders in CdLS patients are a result of dysregulated gene expression. To examine the transcriptional program of Scc2 mutants called scc2-4, RNA sequencing was performed. Analysis of gene expression program shows upregulation of genes involved in ribosome biogenesis and downregulation of genes needed for oxidative phosphorylation. Studies are currently underway to investigate how scc2-4 mutation causes gene misregulation. The answer(s) could provide insight into the molecular etiology of CdLS.
Overall design Examining gene expression in 3 biological replicates of scc2-4 relative to wt by Ilumina sequencing
Contributor(s) Zakari M, Gerton JL, Peak AR, Seidel CW
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Submission date Feb 25, 2014
Last update date May 15, 2019
Contact name Musinu Zakari
Phone 8169264380
Organization name Stowers Institute for Medical Research
Lab Gerton Lab
Street address 1000E 50th Street
City Kansas City
State/province Missouri
ZIP/Postal code 64110
Country USA
Platforms (1)
GPL18330 Illumina HiSeq 2000 (Saccharomyces cerevisiae BY4741)
Samples (9)
GSM1334027 WT rep1 [ribo-depletion]
GSM1334028 WT rep1 [total RNA]
GSM1334029 WT rep2 [ribo-depletion]
This SubSeries is part of SuperSeries:
GSE55358 Genomic analysis of the scc2-4 mutant in budding yeast
BioProject PRJNA239408
SRA SRP038960

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Supplementary file Size Download File type/resource
GSE55316_rpkm.txt.gz 216.1 Kb (ftp)(http) TXT
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Raw data are available in SRA

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