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Series GSE5520 Query DataSets for GSE5520
Status Public on Aug 15, 2006
Title Genome-wide transcriptional analysis of the compatible A. thaliana-P. syringae pv. tomato DC3000 interaction
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Pseudomonas syringae pv. tomato DC3000 (Pst) is a virulent pathogen, which causes disease on tomato and Arabidopsis. The type III secretion system (TTSS) plays a key role in pathogenesis by translocating virulence effectors from the bacteria into the plant host cell, while the phytotoxin coronatine (COR) contributes to virulence and disease symptom development. Recent studies suggest that both the TTSS and and COR are involved in the suppression of host basal defenses. However, little is known about the interplay between the host gene expression associated with basal defenses and the virulence activities of the TTSS and COR during infection. The global effects of the TTSS and COR on host gene expression associated with other host cellular processes during bacterial infection are also not well characterized. In this study, we used the Affymetrix full genome chip to determine the Arabidopsis transcriptome associated with basal defense to Pst DC3000 hrp mutants and the human pathogenic bacterium Escherichia coli O157:H7. We then used Pst DC3000 virulence mutants to characterize Arabidopsis transcriptional responses to the action of hrp-regulated virulence factors (e.g., TTSS and COR) during bacterial infection. Additionally, we used bacterial fliC mutants to assess the role of the PAMP flagellin in induction of basal defense-associated transcriptional responses. In total, our global gene expression analysis identified more than 5000 Arabidopsis genes that are reproducibly regulated more than 2-fold in three independent biological replicates of at least one type of comparison. Regulation of these genes provides a molecular signature for Arabidopsis basal defense to plant and human pathogenic bacteria, and illustrates both common and distinct global virulence effects of the TTSS, COR, and possibly other hrp-regulated virulence factors during Pst DC3000 infection.

Experimenter name = William Underwood
Experimenter phone = 517-353-9182
Experimenter fax = 517-353-9168
Experimenter address = Michigan State University
Experimenter address = 222 Plant Biology Building
Experimenter address = 178 Wilson R.d.
Experimenter address = East Lansing, MI
Experimenter zip/postal_code = 48824
Experimenter country = USA
Keywords: pathogenicity_design
 
Overall design 40 samples were used in this experiment
 
Contributor(s) Underwood W, He S, Townsend H, Emmerson Z, Schildknecht B
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Submission date Aug 15, 2006
Last update date Aug 28, 2018
Contact name Nottingham Arabidopsis Stock Centre (NASC)
E-mail(s) affy@arabidopsis.info
Phone +44 (0)115 951 3237
Fax +44 (0)115 951 3297
URL http://arabidopsis.info/
Organization name Nottingham Arabidopsis Stock Centre (NASC)
Department School of Biosciences, University of Nottingham
Street address Sutton Bonington Campus
City Loughborough
ZIP/Postal code LE12 5RD
Country United Kingdom
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (40)
GSM128648 Underwood_1-1_Cor-10e6-24h_Rep1_ATH1
GSM128649 Underwood_1-2_Cor-10e6-24h_Rep2_ATH1
GSM128650 Underwood_1-3_Cor-10e6-24h_Rep3_ATH1
Relations
BioProject PRJNA96173

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5520_RAW.tar 129.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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