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Series GSE55060 Query DataSets for GSE55060
Status Public on Feb 19, 2015
Title SCML2 Establishes the Male Germline Epigenome
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Gametogenesis is dependent on the expression of germline-specific genes. However, it remains unknown how the germline epigenome is distinctly established from that of somatic lineages. Here we show that genes commonly expressed in somatic lineages and spermatogenesis-progenitor cells undergo repression in a genome-wide manner in late stages of the male germline and identify underlying mechanisms. SCML2, a germline-specific subunit of a Polycomb repressive complex 1 (PRC1), establishes the unique epigenome of the male germline through two distinct antithetical mechanisms. SCML2 works with PRC1 and promotes RNF2-dependent ubiquitination of H2A, thereby marking somatic/progenitor genes on autosomes for repression. Paradoxically, SCML2 also prevents RNF2-dependent ubiquitination of H2A on sex chromosomes during meiosis, thereby enabling unique epigenetic programming of sex chromosomes for male reproduction. Our results reveal divergent mechanisms involving a shared regulator by which the male germline epigenome is distinguished from that of the soma and progenitor cells.
Overall design RNA-seq and ChIP-seq analyses using wild-type and Scml2-KO spermatogenic cells
Contributor(s) Hasegawa K, Sin HS, Maezawa S, Kartashov AV, Alavattam KG, Bacon WC, Barski A, Namekawa SH
Citation(s) 25703348, 26198001
Submission date Feb 14, 2014
Last update date Oct 10, 2019
Contact name Satoshi Namekawa
Phone 513-803-1377
Organization name Cincinnati Children's Hospital Medical Center
Street address 3333 Burnet Ave
City Cincinnati
State/province Ohio
ZIP/Postal code 45229
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (28)
GSM1328920 RNAseq_GS_WT_1
GSM1328921 RNAseq_GS_WT_2
GSM1328922 RNAseq_PS_WT_1
BioProject PRJNA238350
SRA SRP037992

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Supplementary file Size Download File type/resource
GSE55060_RAW.tar 9.3 Mb (http)(custom) TAR (of CSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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