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| Status |
Public on May 02, 2014 |
| Title |
Astrocyte-encoded positional cues maintain sensorimotor circuit integrity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Astrocytes, the most abundant cells in the central nervous system, promote synapse formation and help refine neural connectivity. Although they are allocated to spatially distinct regional domains during development, it is unknown whether region-restricted astrocytes are functionally heterogeneous. Here we show that postnatal spinal cord astrocytes express several region-specific genes, and that ventral astrocyte-encoded Semaphorin3a (Sema3a) is required for proper motor neuron and sensory neuron circuit organization. Loss of astrocyte-encoded Sema3a led to dysregulated α−motor neuron axon initial segment orientation, markedly abnormal synaptic inputs, and selective death of α−but not of adjacent γ−motor neurons. Additionally, a subset of TrkA+ sensory afferents projected to ectopic ventral positions. These findings demonstrate that stable maintenance of a positional cue by developing astrocytes influences multiple aspects of sensorimotor circuit formation. More generally, they suggest that regional astrocyte heterogeneity may help to coordinate postnatal neural circuit refinement.
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| Overall design |
12 total samples consisting of three biological replicates each of flow sorted postnatal day 7 dorsal spinal cord astrocytes, ventral spinal cord astrocytes, dorsal SC non astrocytes, and ventral SC non astrocytes
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| Contributor(s) |
Molofsky AV, Rowitch DH |
| Citation(s) |
24776795 |
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| Submission date |
Feb 14, 2014 |
| Last update date |
Mar 04, 2019 |
| Contact name |
David Rowitch |
| Organization name |
University of California-San Francisco
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| Department |
Institute for Regenerative Medicine
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| Lab |
Rowitch
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| Street address |
35 Medical Center Way, RMB 932D
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM1328847 |
Postnatal day 7 DORSAL spinal cord Aldh1l1-GFP positive astrocytes, biological rep. 1 |
| GSM1328848 |
Postnatal day 7 VENTRAL spinal cord Aldh1l1-GFP positive astrocytes, biological rep. 1 |
| GSM1328849 |
Postnatal day 7 DORSAL spinal cord Aldh1l1-GFP positive astrocytes, biological rep. 2 |
| GSM1328850 |
Postnatal day 7 VENTRAL spinal cord Aldh1l1-GFP positive astrocytes, biological rep. 2 |
| GSM1328851 |
Postnatal day 7 DORSAL spinal cord Aldh1l1-GFP positive astrocytes, biological rep. 3 |
| GSM1328852 |
Postnatal day 7 VENTRAL spinal cord Aldh1l1-GFP positive astrocytes, biological rep. 3 |
| GSM1328853 |
Postnatal day 7 DORSAL spinal cord Aldh1l1-GFP negative NON-astrocytes, biological rep. 1 |
| GSM1328854 |
Postnatal day 7 VENTRAL spinal cord Aldh1l1-GFP negative NON-astrocytes, biological rep. 1 |
| GSM1328855 |
Postnatal day 7 DORSAL spinal cord Aldh1l1-GFP negative NON-astrocytes, biological rep. 2 |
| GSM1328856 |
Postnatal day 7 VENTRAL spinal cord Aldh1l1-GFP negative NON-astrocytes, biological rep. 2 |
| GSM1328857 |
Postnatal day 7 DORSAL spinal cord Aldh1l1-GFP negative NON-astrocytes, biological rep. 3 |
| GSM1328858 |
Postnatal day 7 VENTRAL spinal cord Aldh1l1-GFP negative NON-astrocytes, biological rep. 3 |
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| Relations |
| BioProject |
PRJNA238347 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE55054_ASvsNonAS_ExpressionSet_Stats.txt.gz |
524.7 Kb |
(ftp)(http) |
TXT |
| GSE55054_Dorsal-AS-vs-Ventral-ASnormalized.txt.gz |
5.0 Kb |
(ftp)(http) |
TXT |
| GSE55054_RAW.tar |
48.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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