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Series GSE5499 Query DataSets for GSE5499
Status Public on Sep 14, 2006
Title Identifying transcription factor functions and targets by phenotypic activation
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Mapping transcriptional regulatory networks is difficult because many transcription factors (TFs) are activated only under specific conditions. We describe a generic strategy for identifying genes and pathways induced by individual TFs that does not require knowledge of their normal activation cues. Microarray analysis of 55 yeast TFs that caused a growth phenotype when overexpressed showed that the majority caused increased transcript levels of genes in specific physiological categories, suggesting a mechanism for growth inhibition. Induced genes typically included established targets and genes with consensus promoter motifs, if known, indicating that these data are useful for identifying potential new target genes and binding sites. We identified the sequence 5'-TCACGCAA as a binding sequence for Hms1p, a TF that positively regulates pseudohyphal growth and previously had no known motif. The general strategy outlined here presents a straightforward approach to discovery of TF activities and mapping targets that could be adapted to any organism with transgenic technology.
Keywords: phenotypic activation
Overall design We analyzed 55 TF overexpression strains and 51 non-essential TF deletion mutants by microarray with fluor-reversal replicates. See Chua et al. (2006), PNAS PMID: 16880382 for details.
Web link
Contributor(s) Chua G, Morris QD, Sopko R, Robinson MD, Ryan O, Chan ET, Frey BJ, Andrews BJ, Boone C, Hughes TR
Citation(s) 16880382
Submission date Aug 10, 2006
Last update date Mar 16, 2012
Contact name Esther T Chan
Organization name Celgene Corporation
Street address 1500 Owens Street, Suite 500
City San Francisco
State/province California
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL1229 Hughes Lab Saccharomyces cerevisiae cDNA ORF array
Samples (270)
GSM126606 ABF1 overexpression mutant vs. Empty vector, replicate 1
GSM126607 ABF1 overexpression mutant vs. Empty vector, replicate 2
GSM126608 ADR1 overexpression mutant vs. Empty vector, replicate 1
BioProject PRJNA96143

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5499_RAW.tar 104.9 Mb (http)(custom) TAR (of TXT)

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