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Series GSE54889 Query DataSets for GSE54889
Status Public on May 22, 2014
Title Early B-cell Factor 1 Regulates Adipocyte Morphology and Lipolysis in White Adipose Tissue [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Examination of EBf1 binding by ChIP-seq in differentiated human adipose stromal cell (hASC) pre-adipocyte
Overall design Pre-Adipocytes differentiated in-vitro were fixed in 1% formaldehyde for 15 min at room temperature and quenched for 5 min by adding glycine to a final concentration of 0.125 M. ChIP assays were then performed with custom-made EBF1 antibody or rabbit IgG . ChIP-sequencing libraries were prepared using NEBnext chip-seq library Prep master mix set from 5 ng of anti-EBF1 and anti-IgG ChIP DNA, respectively. Sequence data were generated with Illumina HiSeq 2000 single-read sequencing and aligned against the human genome (hg19, NCBI).
Contributor(s) Gao H, Mejhert N, Rydén M, Arner P
Citation(s) 24856929
Submission date Feb 11, 2014
Last update date May 15, 2019
Contact name hui Gao
Organization name Karolinska Institutet
Department Department of Biosciences and Nutrition
Street address Novum
City Huddinge
ZIP/Postal code 14183
Country Sweden
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (4)
GSM1326218 Control_IgG, replicate1
GSM1326219 anti_EBF1, replicate 1
GSM1326220 Control_IgG, replicate2
This SubSeries is part of SuperSeries:
GSE54890 Early B-cell Factor 1 Regulates Adipocyte Morphology and Lipolysis in White Adipose Tissue
BioProject PRJNA237972
SRA SRP037584

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54889_RAW.tar 432.3 Mb (http)(custom) TAR (of TXT, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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