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Status |
Public on Aug 04, 2014 |
Title |
Transient ER binding and p300 redistribution support a physiological squelching model for immediate ER-repressed genes |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Selective transcriptional activation and repression of genes throughout signaling cascades and development are poorly understood. Transcription factors (TF) orchestrate patterns and magnitude of transcriptional response, but TF action, or inaction, is highly dependent upon TF kinetics, distance from genes, chromatin architecture, and the local occupancy of other TFs. We integrated genomic transcription, chromosome looping, TF binding, and chromatin structure data to analyze the molecular cascade that results from estradiol-induced (E2) signaling in human MCF-7 breast cancer cells and addressed the context-specific nature of gene regulation. We analyzed kinetic ChIP-seq that profiled the master regulator of the E2-mediated response, estrogen receptor (ER), and found that transient ER binding sites are specifically associated with enhancers of repressed genes.
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Overall design |
We performed replicate ChIP-seq experiments prior to estrogen treatment and 2min, 5min, 10min, 40min, and 160min after E2 treatment.
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Contributor(s) |
Guertin MJ |
Citation(s) |
25051172 |
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Submission date |
Feb 10, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Michael Joseph Guertin |
E-mail(s) |
guertin@virginia.edu
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Phone |
(434) 924-0328
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Organization name |
University of Virginia
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Department |
Biochemistry and Molecular Genetics
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Lab |
Guertin
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Street address |
1340 Jefferson Park Ave
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City |
Charlottesville |
State/province |
VA |
ZIP/Postal code |
22903 |
Country |
USA |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (8)
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Relations |
BioProject |
PRJNA237893 |
SRA |
SRP037572 |
Supplementary file |
Size |
Download |
File type/resource |
GSE54855_RAW.tar |
242.3 Mb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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