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Series GSE54855 Query DataSets for GSE54855
Status Public on Aug 04, 2014
Title Transient ER binding and p300 redistribution support a physiological squelching model for immediate ER-repressed genes
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Selective transcriptional activation and repression of genes throughout signaling cascades and development are poorly understood. Transcription factors (TF) orchestrate patterns and magnitude of transcriptional response, but TF action, or inaction, is highly dependent upon TF kinetics, distance from genes, chromatin architecture, and the local occupancy of other TFs. We integrated genomic transcription, chromosome looping, TF binding, and chromatin structure data to analyze the molecular cascade that results from estradiol-induced (E2) signaling in human MCF-7 breast cancer cells and addressed the context-specific nature of gene regulation. We analyzed kinetic ChIP-seq that profiled the master regulator of the E2-mediated response, estrogen receptor (ER), and found that transient ER binding sites are specifically associated with enhancers of repressed genes.
Overall design We performed replicate ChIP-seq experiments prior to estrogen treatment and 2min, 5min, 10min, 40min, and 160min after E2 treatment.
Contributor(s) Guertin MJ
Citation(s) 25051172
Submission date Feb 10, 2014
Last update date May 15, 2019
Contact name Michael Joseph Guertin
Phone (434) 924-0328
Organization name University of Virginia
Department Biochemistry and Molecular Genetics
Lab Guertin
Street address 1340 Jefferson Park Ave
City Charlottesville
State/province VA
ZIP/Postal code 22903
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (8)
GSM1325246 ER_0min_1
GSM1325247 ER_2min_1
GSM1325248 ER_5min_1
BioProject PRJNA237893
SRA SRP037572

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Supplementary file Size Download File type/resource
GSE54855_RAW.tar 242.3 Mb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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